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. 2023 Aug 22;9(9):e19354.
doi: 10.1016/j.heliyon.2023.e19354. eCollection 2023 Sep.

Chitosan neem nanocapsule enhances immunity and disease resistance in nile tilapia (Oreochromis niloticus)

Rowida E Ibrahim  1 , Gehad E Elshopakey  2 , Abdelwahab A Abdelwarith  3 , Elsayed M Younis  3 , Sameh H Ismail  4 , Amany I Ahmed  5 , Mahmoud M El-Saber  6 , Ahmed E Abdelhamid  7 , Simon J Davies  8 , Abdelhakeem El-Murr  1 , Afaf N Abdel Rahman  1
Affiliations

Chitosan neem nanocapsule enhances immunity and disease resistance in nile tilapia (Oreochromis niloticus)

Rowida E Ibrahim et al. Heliyon. .

Abstract

Finding eco-friendly alternatives for antibiotics in treating bacterial diseases affecting the aquaculture sector is essential. Herbal plants are promising alternatives, especially when combined with nanomaterials. Neem (Azadirachta indica) leaves extract was synthesized using a chitosan nanocapsule. Chitosan neem nanocapsule (CNNC) was tested in-vitro and in-vivo against the Aeromonas sobria (A. sobria) challenge in Nile tilapia. A preliminary experiment with 120 Nile tilapia was conducted to determine the therapeutic dose of CNNC, which was established to be 1 mg/L. A treatment study was applied for seven days using 200 fish categorized into four groups (10 fish/replicate: 50 fish/group). The first (control) and second (CNNC) groups were treated with 0 and 1 mg/L CNNC in water without being challenged. The third (A. sobria) and fourth (CNNC + A. sobria) groups were treated with 0 and 1 mg/L CNNC, respectively, and challenged with A. sobria (1 × 107 CFU/mL). Interestingly, CNNC had an in-vitro antibacterial activity against A. sobria; the minimum inhibitory concentration and minimum bactericidal concentration of CNNC against A. sobria were 6.25 and 12.5 mg/mL, respectively. A. sobria challenge caused behavioral alterations, skin hemorrhage, fin rot, and reduced survivability (60%). The infected fish suffered a noticeable elevation in the malondialdehyde level and hepato-renal function markers (aspartate aminotransferase, alanine aminotransferase, and creatinine). Moreover, a clear depletion in the level of the antioxidant and immune indicators (catalase, reduced glutathione, lysozymes, nitric oxide, and complement 3) was obvious in the A. sobria group. Treatment of the A. sobria-challenged fish with 1 mg/L CNNC recovered these parameters and enhanced fish survivability. Overall, CNNC can be used as a new versatile tool at 1 mg/L as a water treatment for combating the A. sobria challenge for sustainable aquaculture production.

Keywords: Aeromonas sobria; Antimicrobial activity; Chitosan neem nanocapsule; Immunity; Nile tilapia.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
The characterization patterns of CNNC: (A) XRD, (B) TEM, and (C and D) AFM 3 D and 2 D.
Fig. 2
Fig. 2
The characterization patterns of CNNC: (A) Isotherm, (B) pore size and pore volume according to the DH method, (C) DLS, and (D) Zeta potential.
Fig. 3
Fig. 3
Inhibition zones (mm) against A. sobria at a concentration of 100 mg/mL of CNNC (A) and chitosan (B).
Fig. 4
Fig. 4
Effect of CNNC as water exposure on clinical observations of experimentally infected O. niloticus with A. sobria for seven days. (A) Fish of the control group or CNNC group demonstrating normal appearance. (B and C) Fish of the A. sobria group demonstrating skin ulcerations (blue arrows), body hemorrhages, and fin rot (yellow arrows). (D) Fish of the CNNC + A. sobria group demonstrating slight fin rot (yellow arrow).
See this image and copyright information in PMC

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