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. 2023 Dec 1:578:112049.
doi: 10.1016/j.mce.2023.112049. Epub 2023 Sep 4.

Application of specific ELISAs for BMP15 and GDF9 to cumulus cell extracts from infertile women

A E Krysta-Matter  1 , A H Riepsamen  2 , S Lien  2 , W Y T Wong  2 , D Richani  2 , S Kilani  1 , C A Harrison  3 , K A Mallitt  4 , W L Ledger  1 , D M Robertson  2 , R B Gilchrist  5
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Free article

Application of specific ELISAs for BMP15 and GDF9 to cumulus cell extracts from infertile women

A E Krysta-Matter et al. Mol Cell Endocrinol. .
Free article

Abstract

Bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) are oocyte-specific paracrine factors which regulate ovarian cumulus cell (CC) functions. This study aimed to investigate if BMP15 and GDF9 bound to CCs can be characterized, quantified, and show an association with IVF outcomes in infertile women. BMP15 and GDF9 ELISAs were validated and applied to discarded CC extracts. Pooled CCs from individual patients were collected from 120 (cohort 1; BMP15 only) and 81 infertility patients (cohort 2; BMP15 and GDF9) undergoing superovulation. BMP15 and GDF9 levels expressed per CC DNA were correlated with maternal age, clinical and embryology data. Total BMP15 and GDF9 were highly correlated with each other (r = 0.9, p < 0.001). The GDF9:BMP15 ratio was unrelated to oocyte number or age. BMP15/CC DNA and GDF9/CC DNA were unaffected by the type of superovulation and were not related to oocyte/embryo outcomes.

Keywords: Bone morphogenetic protein 15; Cumulin; Cumulus cells; Growth differentiating factor 9; Oocyte.

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Conflict of interest statement

Declaration of competing interest AHR, WLL, DMR and RBG are inventors on a patent, and are co-directors and shareholders of Anlagen Pty. Ltd., which are relevant to this work. All other authors have nothing to disclose.

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