Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Sep 5;39(11):299.
doi: 10.1007/s11274-023-03749-3.

Biofilm formation by agave epiphytic lactic acid bacteria fed with agave fructans

Nayeli Martha-Lucero  1 , Gustavo Viniegra-González  1 , Luis González-Olivares  2 , Alma Cruz-Guerrero  3
Affiliations

Biofilm formation by agave epiphytic lactic acid bacteria fed with agave fructans

Nayeli Martha-Lucero et al. World J Microbiol Biotechnol. .

Abstract

The aim of this work was to find out if biofilms can be made by lactic acid bacteria (LAB) isolated from agave plants using agave fructans as sole carbohydrate substrates or if it was necessary to use fructose as a breakdown product of such polymers. This is part of a research project geared to develop industrial lactic acid production from agave fructans, an abundant raw material in Mexico's agave plantations. Present results showed that nine strains of LAB isolated from Agave salmiana and belonging to genus Lacticaseibacillus and Enterococcus produced exopolysaccharides directly from agave fructans to a greater extent than with fructose. The best polysaccharide productions in planktonic cultures were Lacticaseibacillus paracasei strains DG2, DG3, DG4 and DG8. Furthermore, all nine LAB strains produced biofilms on polystyrene microplates, much better with agave fructans than with fructose. In most strains, biofilm formation was favored at pH from 6.0 to 6.5, except for strains DG7 and DG9 where pH 5.5 was optimal. Biofilm formation required between 3 and 5 days of incubation in all Lacticaseibacillus paracasei strains, whereas Enterococcus faecium required a little less of 3 days. Present results support the straight use of agave fructans to develop LAB biofilms using agave epiphytic bacteria. This finding simplifies upstream processing of agave fructans to be used for future lactic acid fermentation in LAB biofilm reactors.

Keywords: Agave fructans; Biofilm; Enterococcus; Lactic acid bacteria; Lacticaseibacillus.

PubMed Disclaimer

References

    1. Akoğlu A (2020) The effect of some environmental conditions on planktonic growth and biofilm formation by some lactic acid bacteria isolated from a local cheese in Turkey. Biotechnol Lett 42:481–492. https://doi.org/10.1007/s10529-020-02794-4 - DOI - PubMed
    1. Ávila-Fernández Á, Rendón-Poujol X, Olvera C, González F, Capella S, Peña-Álvarez A, López-Munguía A (2009) Enzymatic hydrolysis of fructans in the tequila production process. J Agric Food Chem 57:5578–5585. https://doi.org/10.1021/jf900691r - DOI - PubMed
    1. Awad AI, Ahmaed AS (2018) Studying best conditions for biofilm production from Leuconostoc mesenteroides. J Res Ecology 6:2185–2191
    1. Ayala-Monter MA, Hernández-Sánchez D, Pinto-Ruiz R, González-Muñoz SS, Bárcena-Gama JR, Hernández-Mendo O, Torres-Salado N (2018) Efecto prebiótico de dos fuentes de inulina en el crecimiento in vitro de Lactobacillus salivarius y Enterococcus faecium. Rev Mex Cienc Pecu 9:346–361. https://doi.org/10.22319/rmcp.v9i2.4488 - DOI
    1. Bastarrachea LJ, Britt DW, Ward RE, Demirci A (2022) Development of bioactive solid support for immobilized Lactobacillus casei biofilms and the production of lactic acid. Bioprocess Biosyst Eng 45:217–226. https://doi.org/10.1007/s00449-021-02654-z - DOI - PubMed

LinkOut - more resources