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. 2024 Jan;78(1):216-225.
doi: 10.1007/s11418-023-01740-8. Epub 2023 Sep 5.

Isovincathicine from Catharanthus roseus induces apoptosis in A549 cells

Mizuki Hiroki  1 , Aishanjiang Abulikemu  1 , Chihiro Totsuka  1 , Yusuke Hirasawa  2 , Toshio Kaneda  1 , Hiroshi Morita  3
Affiliations

Isovincathicine from Catharanthus roseus induces apoptosis in A549 cells

Mizuki Hiroki et al. J Nat Med. 2024 Jan.

Abstract

A dimeric indole alkaloid, isovincathicine consisting of an aspidosperma type and modified iboga with C-7-C-20 connection type skeletons was first isolated from Catharanthus roseus, and the structure including stereochemistry was elucidated on the basis of spectroscopic data as well as DP4 statistical analysis. Isovincathicine inhibited cell proliferation in A549 cells. We investigated the detailed mode of action of isovincathicine-induced inhibitory effects on cell proliferation in A549 cells. Flow cytometric analysis showed that isovincathicine-treated cells accumulated in the G2 phase after 24 h, and the percentage of cells showing cell death increased after 48 h. Western blotting also showed increased expression of BimEL, an apoptosis-related protein, and decreased expression of Mcl-1 and Bcl-xL. Isovincathicine was suggested to induce apoptosis in A549 cells by a mechanism is similar to that of vinblastine.

Keywords: Apoptosis; Bisindole alkaloid; Catharanthus roseus; Cell proliferation; Cytotoxic activity; DP4 statistical analysis; Isovincathicine.

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