Ultrastructural observations on nucleoli and related structures during human spermatogenesis

Abstract

The ultrastructural study of nucleoli and ribonucleoprotein-containing structures in human seminiferous tubules revealed that the nucleoli of spermatogonia, spermatocytes and Sertoli cells exhibited a tripartite structure consisting of: a fibrillar center, a compact granular portion, and a reticular portion containing both pars fibrosa and pars granulosa. The nucleoli of primary spermatocytes showed a developed reticular portion. At pachytene, the compact granular portion enlarged and lost its connection with the fibrillar center and the reticular portion which decreased in size. This suggests a nucleolar segregation similar to that of ovocytes in many species. Two similar developmental stages of nucleoli were observed in spermatogonia. In addition to nucleoli, there were other ribonucleoprotein-containing structures such as intranuclear closely-packed granules in Ap spermatogonia, coarse granules in the chromatin rarefaction zone of Ad spermatogonia, the nuage and Lubarsch crystals of spermatogonia, the chromatoid body of spermatids, the annulate lamellae of both spermatids and Sertoli cells, and many structures of the spermatid neck region.