Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Sep 6;21(1):91.
doi: 10.1186/s43141-023-00543-4.

Use of ISSR markers to assess the genetic diversity of an endemic plant of Morocco (Euphorbia resinifera O. Berg)

Hassane Abd-Dada  1 , Said Bouda  1 , Youssef Khachtib  1 , Youssef Ait Bella  1 , Abdelmajid Haddioui  2
Affiliations

Use of ISSR markers to assess the genetic diversity of an endemic plant of Morocco (Euphorbia resinifera O. Berg)

Hassane Abd-Dada et al. J Genet Eng Biotechnol. .

Abstract

Background: Euphorbia resinifera is a melliferous, medicinal, and endemic plant to Morocco. Nevertheless, its ecological and genetic diversity still unknown. The objective of this study is to analyze the diversity and genetic structure of Moroccan wild populations of E. resinifera using ISSR markers. Twelve natural populations collected from its geographical range in Morocco were analyzed using 14 ISSR primers.

Results: A total of 125 bands were obtained, with polymorphism of 74.81%. The polymorphic information content (PIC), resolving power (Rp), Shannon's information index (I), and total genetic diversity (Ht) were 0.33, 2.8, 0.35, and 0.21, respectively. The analysis of molecular variance showed that 75.56% of the total variability is present within populations and that 24.44% exists among populations. Also, the analysis showed a very low genetic differentiation between groups of mountain range type (FCT = 0.066), mountain versant type groups (FCT = -0.024), and altitude groups (FCT = -0.022). Moreover, the geographical distances between populations are correlated with their corresponding genetic distances according to the Mantel test (r = 0.507; P < 0.0001).

Conclusion: These results suggest that the population structuring follows a model of isolation by geographical distance. Indeed, the genetic structuring of populations into two groups obtained from PCoA and structure analysis revealed a dependence on the geographical origin of the populations. By contrast, the genetic distances are not correlated with the altitude.

Keywords: Atlas Mountain; Euphorbia resinifera; Genetic diversity; ISSR; Polymorphism.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Map of Morocco showing locations of the E. resinifera populations studied
Fig. 2
Fig. 2
Bar plots represent STRUCTURE inferences of individual assignments (N = 60, K = 2, ΔK = 13.71, H’ = 0.947). Each individual is represented by a vertical bar showing membership coefficients to each genetic cluster
Fig. 3
Fig. 3
PCoA plot of the E. resinifera individuals showing relationships based on ISSR data
See this image and copyright information in PMC

References

    1. Horn JW, Xi Z, Riina R, Peirson JA, Yang Y, Dorsey BL, Berry PE, Davis CC, Wurdack KJ. Evolutionary bursts in Euphorbia (Euphorbiaceae) are linked with photosynthetic pathway. Evolution. 2014;68(12):3485–3504. doi: 10.1111/evo.12534. - DOI - PubMed
    1. Govaerts R, Frodin DG, Radcliffe-Smith A. World checklist and bibliography of Euphorbiaceae (and Pandaceae) R Bot Gard Kew. 2000;1–4:1–1622.
    1. Abd-dada H, Bouda S, Ait bella Y, Haddioui A. Phenotypic diversity of natural populations of an endemic Moroccan plant (Euphorbia resinifera O. Berg) Hacquetia. 2023;22(1):81–90. doi: 10.2478/hacq-2022-0015. - DOI
    1. Steinmann VW, Porter JM. Phylogenetic relationships in Euphorbieae (Euphorbiaceae) based on its and ndhF sequence data. Ann Mo Bot Gard. 2002;89:453–490. doi: 10.2307/3298591. - DOI
    1. Jahandiez É, Maire R (1932) Catalogue des Plantes du Maroc: Spermaphytes et Ptéridophytes. Tome deuxième: Dicotylédones Archichlamydées 2, Alger, p 461. https://bibdigital.rjb.csic.es/viewer/13175/?offset=#page=305&viewer=pic...

LinkOut - more resources