Evaluation of ethanol-induced chitosan aerogels with human osteoblast cells

Abstract

The following article provides an insight into the production of chitosan aerogels as potential materials for tissue engineering. Chitosan aerogels were prepared following two different protocols: formation in ethanol and formation in sodium hydroxide in an ethanol solution. The main objective was to apply a new route to obtain chitosan aerogels with no external cross-linkers and compare the mentioned preparation approaches. Forming chitosan aerogels in ethanol implies a simple, environmentally friendly, and efficient method. The prepared materials showed specific surface areas of up to 450 m²/g, highly porous networks and great mechanical properties. In vitro degradation studies revealed high stability for up to 10 weeks. The differences between the samples were significant. While the chitosan aerogels prepared in ethanol showed superior textural, morphological and mechanical properties, the chitosan aerogels prepared in the sodium hydroxide solution proved that a considerable influence on end properties could be made simply by adjusting the ageing medium. In vitro cell analysis with primary human osteoblasts showed good biocompatibility and pointed towards the potential use of these aerogels for orthopedic applications. This testing showed further that adjustments in structural properties by sodium hydroxide also come with a cost regarding their suitability to host bone cells.

Keywords: Aerogels; Bone tissue engineering; Chitosan; Osteoblasts; Supercritical drying.