Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979;26(3):229-44.
doi: 10.1016/0022-1759(79)90248-5.

A quantitative assay for low levels of IgM by solid-phase immunofluorescence

J T Sundeen, R S Krakauer

A quantitative assay for low levels of IgM by solid-phase immunofluorescence

J T Sundeen et al. J Immunol Methods. 1979.

Abstract

A quantitative immunofluorescent assay capable of detecting between 15 and 1450 ng of IgM on a strictly linear standard curve has been developed. The assay system is unique in that in vitro biosynthesized polyclonal IgM in culture supernatants can be quantitated with a minimal quantity of supernatant. Interference created by lectins and bead-surface interactions have been analyzed. This easily operated assay has been validated and is reliable. A very important feature of the system is the considerable cost reduction in comparsion with radiometric techniques.

PubMed Disclaimer

MeSH terms

Substances

LinkOut - more resources