Corrigendum: Dynamics of host immune response development during Schistosoma mansoni infection

Abstract

[This corrects the article DOI: 10.3389/fimmu.2022.906338.].

Keywords: chronic infection; dendritic cells; pathology; schistosomiasis; transcriptomic (RNA-seq).

Figure 2

Tissue-specific cellular responses during schistosomiasis. Stacked bar charts showing hepatic splenic and mesenteric (A) cell counts and (B) cell frequencies (as a proportion of total live cells) at indicated wks of infection when infected with 40 cercariae. For infected mice, data is presented as mean values for each given time point, with averages calculated from two pooled experiments per time point. n=6-8 per timepoint from two pooled experiments. Naïve data is presented as mean values for the entire infection, with averages calculated from two pooled time course experiments. n=30. Significance in (A) reflects comparison of total cell counts between naïve and infected mice. Statistics tables showing differences in (C) cell counts and (D) cell frequencies between naïve and infected mice, for the liver spleen and MLN. Arrows in table (C, D) represent the direction of cell frequency change in infected animals in comparison to naïve. Significance calculated by Kruskal-Wallis followed by Dunn’s multiple comparisons test, with comparison between naïve and infected groups. *p < 0.05, **p < 0.01, ***p < 0.001, ns = non-significant (P > 0.05).

Figure 6

CD11c depletion disrupts granulomatous pathology during S. mansoni infection. (A) Schematic of infection setup. CD11c.DOG mice were infected with 40 S. mansoni cercariae with CD11c⁺ cells depleted via Dtx administration on days 42-51, and mice culled at d52. (B) Liver and spleen weights for infected mice with data represented as a proportion of total body weight. (C) The total number of schistosome eggs per gram of liver or intestinal tissue. (D) Quantification of granulomatous inflammation. (E) Representative images of hepatic granulomas stained with H&E. (F) Representative confocal microscopy granuloma images, with staining for CD11c, Siglec-F and TCRβ. First column showing differential Interference Contrast (DIC) images, with eggs indicated by arrows and dotted lines outlining granuloma periphery. (G) Quantification of positive Siglec-F, CD11C and TCRβ staining. Data are from a single experiment (D-G) or pooled from 3 (A–C) 3 separate experiments. Significance calculated by unpaired T-test. Data presented as mean +/- SEM. *p < 0.05, **p < 0.01, ***p < 0.001.