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Review
. 2023:2702:247-260.
doi: 10.1007/978-1-0716-3381-6_12.

Antibody Selection via Phage Display in Microtiter Plates

Stephan Steinke  1 , Kristian Daniel Ralph Roth  1 , Maximilian Ruschig  1 , Nora Langreder  1 , Saskia Polten  1 , Kai-Thomas Schneider  1 , Rico Ballmann  1 , Giulio Russo  1 , Kilian Johannes Karl Zilkens  2 , Maren Schubert  1 , Federico Bertoglio  1   3 , Michael Hust  4
Affiliations
Review

Antibody Selection via Phage Display in Microtiter Plates

Stephan Steinke et al. Methods Mol Biol. 2023.

Abstract

The most common and robust in vitro technology to generate monoclonal human antibodies is phage display. This technology is a widely used and powerful key technology for recombinant antibody selection. Phage display-derived antibodies are used as research tools, in diagnostic assays, and by 2022, 14 phage display-derived therapeutic antibodies were approved. In this review, we describe a fast high-throughput antibody (scFv) selection procedure in 96-well microtiter plates. The given detailed protocol allows the antibody selection ("panning"), screening, and identification of monoclonal antibodies in less than 2 weeks. Furthermore, we describe an on-rate panning approach for the selection of monoclonal antibodies with fast on-rates.

Keywords: Antibody fragments; Antibody phage display; Antibody selection; Microtiter plates (MTPs); Monoclonal antibodies; On-rate panning; Panning; Phage display; scFv.

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