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. 2023 Aug 23:10:1194242.
doi: 10.3389/fvets.2023.1194242. eCollection 2023.

Evaluation of thrombin generation in dogs administered clopidogrel

Kaitlyn Rank  1 , Alex M Lynch  1 , Laura K Ruterbories  1 , Ronald H L Li  1   2 , Yu Ueda  1
Affiliations

Evaluation of thrombin generation in dogs administered clopidogrel

Kaitlyn Rank et al. Front Vet Sci. .

Abstract

Introduction: The antiplatelet effect of clopidogrel can vary between patients. A modified thromboelastography (TEG) protocol (TEG-Platelet Mapping assay® [TEG-PM]) can be used for clopidogrel monitoring but is not widely available. Thrombin generation (TG) assays could offer a novel alternative. The main objective of this pilot study was to assess TG assay variables (lag time, peak, endogenous thrombin potential [ETP]) in dogs before and after 7 days of clopidogrel administration and compare with TEG-PM variables (maximum amplitude [MA]-ADP and percentage (%) inhibition).

Methods: Six healthy mix-breed dogs were enrolled in this pilot study. Blood samples for platelet count, TG assays, and TEG-PM were obtained at two time points, corresponding to baseline, and after 7 days of clopidogrel administration (mean 2.3 +/- 0.3 mg/kg PO q24 hours). Data were then compared with a Student's t-test.

Results: There was no significant change in TG assay variables performed on platelet poor plasma after 7 days of clopidogrel administration: lag time (Day 1: 1.8 +/- 0.2 min, Day 7: 1.8 +/- 0.2 min, p = 0.42); peak (Day 1: 76 +/- 7 nM, Day 7: 72 +/- 10 nM, p = 0.49); and ETP (Day 1: 399 +/- 27 nM*min, Day 7: 392 +/- 32 nM*min; p = 0.49). There were significant changes in TEG MA-ADP (Day 1: 19 +/- 8 mm, Day 7: 9 +/- 6 mm, p = 0.04) and % inhibition (Day 1: 58 +/- 27, Day 7: 99 +/- 0.3, p = 0.02).

Discussion: Clopidogrel administration did not lead to changes in TG assay variables performed on platelet poor plasma samples, despite concomitant changes in TEG-PM variables consistent with platelet inhibition. Based on this pilot study, thrombin generation performed on platelet poor plasma may not be a useful antiplatelet monitoring tool in dogs.

Keywords: antithrombotic; canine; coagulation; platelet inhibitory; therapeutic monitoring.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The reviewer CB declared a past co-authorship with the author AL to the handling editor.

Figures

Figure 1
Figure 1
Correlations between the TEG-PM variable (MA-ADP) and TG-variables, specifically (A) MA-ADP (mm) vs. Lag time (min); (B) MA-ADP vs. Peak (nM); (C) MA-ADP vs. ETP (nM*min). Significance was set at a P-value <0.05. ETP, endogenous thrombin potential; MA, maximum amplitude; TEG-PM, TEG Platelet Mapping®; TG, thrombin generation.
See this image and copyright information in PMC

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