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. 2023 Aug 30:2023:9942842.
doi: 10.1155/2023/9942842. eCollection 2023.

Real-World Study on Chai-Shi-Jie-Du Granules for the Treatment of Dengue Fever and the Possible Mechanisms Based on Network Pharmacology

Huiqin Yang  1 , Dehong Ma  2 , Qin Li  3 , Wen Zhou  3 , Hongyi Chen  4 , Xiyun Shan  2 , Haipeng Zheng  1 , Chun Luo  5 , Zhiyue Ou  6 , Jielan Xu  6 , Changtai Wang  6 , Lingzhai Zhao  7 , Rui Su  8 , Yuehong Chen  9 , Qingquan Liu  8 , Xinghua Tan  5 , Luping Lin  5 , Tao Jiang  9 , Fuchun Zhang  1
Affiliations

Real-World Study on Chai-Shi-Jie-Du Granules for the Treatment of Dengue Fever and the Possible Mechanisms Based on Network Pharmacology

Huiqin Yang et al. Evid Based Complement Alternat Med. .

Abstract

Objectives: Traditional Chinese medicine (TCM) is a widely used method for treating dengue fever in China. TCM improves the symptoms of patients with dengue, but there is no standard TCM prescription for dengue fever. This real-world study aimed to evaluate the effects of Chai-Shi-Jie-Du (CSJD) granules for the treatment of dengue fever and the underlying mechanisms.

Methods: We implemented a multicenter real-world study, an in vitro assay and network pharmacology analysis. Patients from 5 hospitals in mainland China who received supportive western treatment in the absence or presence of CSJD were assigned to the control and CSJD groups between 1 August and 31 December 2019. Propensity score matching (PSM) was performed to correct for biases between groups. The clinical data were compared and analyzed. The antidengue virus activity of CSJD was tested in Syrian baby hamster kidney (BHK) cells using the DENV2-NGC strain. Network pharmacological approaches along with active compound screening, target prediction, and GO and KEGG enrichment analyses were used to explore the underlying molecular mechanisms.

Results: 137 pairs of patients were successfully matched according to age, sex, and the time from onset to presentation. The time to defervescence (1.7 days vs. 2.5 days, P < 0.05) and the disease course (4.1 days vs. 6.1 days, P < 0.05) were significantly shorter in the CSJD group than those in the control group. CSJD showed no anti-DENV2-NGC virus activity in BHK cells. Network pharmacology analysis revealed 108 potential therapeutic targets, and the top GO and KEGG terms were related to immunity, oxidative stress response, and the response to lipopolysaccharide.

Conclusions: CSJD granules exhibit high potential for the treatment of dengue fever, and the therapeutic mechanisms involved could be related to regulating immunity, moderating the oxidative stress response, and the response to lipopolysaccharide.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Flowchart of the participants. TCM, traditional Chinese medicine. CSJD, Chai-Shi-Jie-Du granules. PSM, propensity score matching.
Figure 2
Figure 2
Comparison of the time to defervescence and the disease course between the CSJD and the control groups.
Figure 3
Figure 3
Cytotoxic effect and antiviral activity of CSJD granules on BHK cells. (a) Cytotoxic effect of CSJD; (b) antiviral activity of CSJD granules against DENV-2. Data are presented as the mean ± SD.
Figure 4
Figure 4
(a) Active compounds and targets of constituents in CSJD granules. (b) A total of 794 and 956 targets of CSJD and dengue fever, respectively, with 108 shared targets.
Figure 5
Figure 5
(a) The top terms of GO enrichment of BP and MF (P < 0.05). (b) The top 30 pathways identified in the KEGG enrichment analysis (P < 0.05).
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