Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2023 Aug 29;13(17):2449.
doi: 10.3390/nano13172449.

Recent Advances in DNA Nanomaterials

Incherah Bekkouche  1 , Maria N Kuznetsova  1 , Dovlet T Rejepov  1 , Alexandre A Vetcher  1   2 , Alexander Y Shishonin  2
Affiliations
Review

Recent Advances in DNA Nanomaterials

Incherah Bekkouche et al. Nanomaterials (Basel). .

Abstract

Applications of DNA-containing nanomaterials (DNA-NMs) in science and technology are currently attracting increasing attention in the fields of medicine, environment, engineering, etc. Such objects have become important for various branches of science and industries due to their outstanding characteristics such as small size, high controllability, clustering actions, and strong permeability. For these reasons, DNA-NMs deserve a review with respect to their recent advancements. On the other hand, precise cluster control, targeted drug distribution in vivo, and cellular micro-nano operation remain as problems. This review summarizes the recent progress in DNA-NMs and their crossover and integration into multiple disciplines (including in vivo/in vitro, microcircles excisions, and plasmid oligomers). We hope that this review will motivate relevant practitioners to generate new research perspectives and boost the advancement of nanomanipulation.

Keywords: DNA; aptamers; microcircles; nanomaterials; plasmid.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Biomedical nanotechnology diagram.
Figure 2
Figure 2
Schematic representation of the two primary design methods currently used to create DNA-NMs: (a) the tile-based; (b) the scaffold-based. The colored curved lines represent partially complementes oligos, while arrows point the oligos direction.
Figure 3
Figure 3
Improvement techniques for DNA-NMs and aptamers’ stability. (a) By ligating the 30 and 50 ends of the bivalent aptamers, a circle is created (1–3—steps of the process). (b) The use of fluorinated bases as useful nano molecules to enhance the stability of molecular beacons. (c) The new base pair pattern (zT-A) and the photosensitive base (zT). (d) PEGylated oligolysine-coated DNA-NMs with strong nuclease resistance are crosslinked with glutaraldehyde.
Figure 4
Figure 4
Microcircles generation with Cre recombinase. (a) The recombination either from two direct or inverted loxP sites (shown by jade arrows) results in excised microcircle. (b) AFM image of 146 bp microcircles from A.A.V.’s collection. The scale bar is 50 nm in length. The dotted arrow points to the microcircle.
Figure 5
Figure 5
SWNTs and single-stranded DNA (ssDNA) have been combined in a theoretical model.
See this image and copyright information in PMC

References

    1. Du S.M., Zhang S., Seeman N.C. DNA junctions, antijunctions, and mesojunctions. Biochemistry. 1992;31:10955–10963. doi: 10.1021/bi00160a003. - DOI - PubMed
    1. Zheng J., Birktoft J.J., Chen Y., Wang T., Sha R., Constantinou P.E., Ginell S.L., Mao C., Seeman N.C. From molecular to macroscopic via the rational design of a self-assembled 3D DNA crystal. Nature. 2009;461:74–77. doi: 10.1038/nature08274. - DOI - PMC - PubMed
    1. Zhang D.Y., Seelig G. Dynamic DNA nanotechnology using strand-displacement reactions. Nat. Chem. 2011;3:103–113. doi: 10.1038/nchem.957. - DOI - PubMed
    1. Endo M., Yang Y., Suzuki Y., Hidaka K., Sugiyama H. Single-Molecule Visualization of the Hybridization and Dissociation of Photoresponsive Oligonucleotides and Their Reversible Switching Behavior in a DNA Nanostructure. Angew. Chem. Int. Ed. 2012;51:10518–10522. doi: 10.1002/anie.201205247. - DOI - PubMed
    1. Liedl T., Simmel F.C. Switching the Conformation of a DNA Molecule with a Chemical Oscillator. Nano Lett. 2005;5:1894–1898. doi: 10.1021/nl051180j. - DOI - PubMed

LinkOut - more resources