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. 2023 Aug 25;12(17):3063.
doi: 10.3390/plants12173063.

Rosehip Extract-Loaded Liposomes for Potential Skin Application: Physicochemical Properties of Non- and UV-Irradiated Liposomes

Aleksandra A Jovanović  1 , Bojana Balanč  2 , Mina Volić  2 , Ilinka Pećinar  3 , Jelena Živković  4 , Katarina P Šavikin  4
Affiliations

Rosehip Extract-Loaded Liposomes for Potential Skin Application: Physicochemical Properties of Non- and UV-Irradiated Liposomes

Aleksandra A Jovanović et al. Plants (Basel). .

Abstract

In the present study, rosehip (Rosa canina L.) extract was successfully encapsulated in phospholipid liposomes using a single-step procedure named the proliposome method. Part of the obtained liposomes was subjected to UV irradiation and non-treated (native) and UV-irradiated liposomes were further characterized in terms of encapsulation efficiency, chemical composition (HPLC analysis), antioxidant capacity, particle size, PDI, zeta potential, conductivity, mobility, and antioxidant capacity. Raman spectroscopy as well as DSC analysis were applied to evaluate the influence of UV irradiation on the physicochemical properties of liposomes. The encapsulation efficiency of extract-loaded liposomes was higher than 90%; the average size was 251.5 nm; the zeta potential was -22.4 mV; and the conductivity was found to be 0.007 mS/cm. UV irradiation did not cause a change in the mentioned parameters. In addition, irradiation did not affect the antioxidant potential of the liposome-extract system. Raman spectroscopy indicated that the extract was completely covered by the lipid membrane during liposome entrapment, and the peroxidation process was minimized by the presence of rosehip extract in liposomes. These results may guide the potential application of rosehip extract-loaded liposomes in the food, pharmaceutical, or cosmetic industries, particularly when liposomal sterilization is needed.

Keywords: Raman spectroscopy; Rosa canina; UV irradiation; encapsulation; liposomes; proliposome method.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Antioxidant capacity of rosehip extract and of empty and extract-loaded liposomes before and after UV irradiation, and ascorbic acid (as a control, at a concentration of 0.2 mg/mL) determined in ABTS and DPPH assays; different letters (a–j) indicate that there was a statistically significant difference based on Duncan’s post hoc test at p < 0.05 level, n = 3, mean value ± standard deviation.
Figure 2
Figure 2
Averages of normalized Raman spectra of rosehip extract (A), Phospholipon (B), liposomes (C), liposomes treated with UV irradiation (D), liposomes with extract (E), and liposomes with extract treated with UV irradiation (F), in the spectral range from 250 to 3500 cm−1.
Figure 3
Figure 3
Differential scanning calorimetry curves of rosehip extract-loaded liposomes, unloaded liposomes, pure extract (UV-irradiated and non-treated samples), and pure Phospholipon.
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