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. 2023 Nov 1;133(21):e171982.
doi: 10.1172/JCI171982.

IRGM1 supports host defense against intracellular bacteria through suppression of type I interferon in mice

Prashant Rai  1 , Martin Sharpe  1 , Charan K Ganta  2 , Paul J Baker  3 , Katrin D Mayer-Barber  3 , Brian E Fee  4 , Gregory A Taylor  4   5 , Michael B Fessler  1
Affiliations

IRGM1 supports host defense against intracellular bacteria through suppression of type I interferon in mice

Prashant Rai et al. J Clin Invest. .
No abstract available

Keywords: Bacterial infections; Immunology; Infectious disease; Innate immunity; Macrophages.

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Conflict of interest statement

Conflict of interest: The authors have declared that no conflict of interest exists.

Figures

Figure 1
Figure 1. IFN-I induces susceptibility to bacterial infection in Irgm1–/– mice.
(A) Survival curves after infection with M. tuberculosis (n = 7–10) and L. monocytogenes (n = 4–5). Spleen CFU on day 21 after S. typhimurium infection (n = 4–9). (B) Liver on day 3 after L. monocytogenes infection stained for TUNEL and DAPI. Scale bar: 200 μm. (C) Quantification of TUNEL+ foci (n = 3). (D) Peritoneal lavage CFU at 4 hours, day 1, and day 3 after L. monocytogenes (n = 4–8). (E) Isolated F4/80hi peritoneal macrophages exposed to L. monocytogenes were permeabilized for CFU count after 24 hours. (F) Macrophages were stained for L. monocytogenes and lysosome (LAMP1) at 6 hours and quantified for volumetric pixels of L. monocytogenes that were LAMP1+ (n = 32 images). (G) Percentage GFP+ after 4-hour infection by GFP-tagged L. monocytogenes (n = 9–10). (H) Neutrophil and (I) F4/80hi tissue macrophage numbers in infected peritoneal lavage (n = 7–11). (J) Representative plot showing depletion of CD11b+F4/80hi macrophages at 24 hours. (K) CFU in peritoneal cavity (PerC), liver, and spleen on day 3 after L. monocytogenes (n = 5–8). Data are shown as the mean ± SEM. #P < 0.08, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (1-way ANOVA with Tukey’s adjustment for A and C–I or Student’s t test for K).
See this image and copyright information in PMC

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