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. 2023 Aug 28:14:1213494.
doi: 10.3389/fpls.2023.1213494. eCollection 2023.

Transcriptome analysis of two tobacco varieties with contrast resistance to Meloidogyne incognita in response to PVY MSNR infection

Shixiao Xu  1 , Pei Tian  2 , Zhimin Jiang  3 , Xiaoxiang Chen  3 , Bo Li  3 , Jutao Sun  1 , Zhiqiang Zhang  1
Affiliations

Transcriptome analysis of two tobacco varieties with contrast resistance to Meloidogyne incognita in response to PVY MSNR infection

Shixiao Xu et al. Front Plant Sci. .

Abstract

Root-knot nematode (RKN) disease is a major disease of tobacco worldwide, which seriously hinders the improvement of tobacco yield and quality. Obvious veinal necrosis-hypersensitive responses are observed only in RKN-resistant lines infected by Potyvirus Y (PVY) MSNR, making this an effective approach to screen for RKN-resistant tobacco. RNA-seq analysis, real-time quantitative PCR (qRT-PCR) and functional enrichment analysis were conducted to gain insight into the transcription dynamics difference between G28 (RKN-resistant) and CBH (RKN-susceptible) varieties infected with PVY MSNR. Results showed that a total of 7900, 10576, 9921, 11530 and 12531 differentially expressed genes (DEGs) were identified between the two varieties at 0, 1, 3, 5, and 7 d after infection, respectively. DEGs were associated with plant hormone signal transduction, starch and sucrose metabolism, phenylpropanoid biosynthesis, and photosynthesis-related metabolic pathways. Additional DEGs related to starch and sucrose metabolism, energy production, and the indole-3-acetic acid signaling pathway were induced in CBH plants after infection. DEGs related to phenylpropanoid biosynthesis, abscisic acid, salicylic acid, brassinosteroids, and jasmonic acid signaling pathway were induced in G28 after infection. Our findings reveal DEGs that may contribute to differences in PVY MSNR resistance among tobacco varieties. These results help us to understand the differences in transcriptional dynamics and metabolic processes between RKN-resistant and RKN-susceptible varieties involved in tobacco-PVY MSNR interaction.

Keywords: PVY MSNR; resistance; root-knot nematode; tobacco; transcriptome.

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Conflict of interest statement

Authors SX, JS, and ZZ are employed by the College of Tobacco Science, Henan Agricultural University, National Tobacco Cultivation and Physiology and Biochemistry Research Centre, Scientific Observation and Experiment Station of Henan, Ministry of Agriculture. Author PT is employed by China Tobacco Jiangsu Industry Co, Ltd. Xuzhou Cigarette Factory. Authors ZJ, XC, and BL are employed by China Tobacco Zhejiang Industry Co, Ltd.

Figures

Figure 1
Figure 1
Phenotypic and physiological changes after infection of G28 and CBH tobacco leaves with PVY MSNR. (A) G28 leaf before infection. (B) G28 leaf after infection for 7 d. (C) CBH leaf before infection. (D) CBH leaf after infection for 7 d. (E–H) DAB staining of CBH leaves after infection for 1, 3, 5, and 7 d. (I–L) DAB staining of G28 leaves after infection for 1, 3, 5, and 7 d.
Figure 2
Figure 2
Overview of the transcriptomes of G28 and CBH tobacco varieties after infection with PVY MSNR. (A) Expression density of genes in the two varieties after infection. (B) Principal component analysis of genes identified from 30 samples analyzed by RNA-seq.
Figure 3
Figure 3
Expression trend analysis of expressed genes after infection of G28 and CBH tobacco varieties with PVY MSNR. The top number represents the total number of genes in each profile. The bottom number represents the p-value. The line represents the gene expression trends of each profile.
Figure 4
Figure 4
Numbers of specific DEGs in different comparison sets after infection of G28 and CBH tobacco varieties with PVY MSNR. (A) Numbers of up- and down-regulated DEGs. (B) Venn diagrams highlighting unique DEGs.
Figure 5
Figure 5
Functional analysis of DEGs in various comparison groups. KEGG pathway analysis of DEGs in (A) CBH and (B) G28 after 1, 3, 5, and 7 d infection with PVY MSNR compared with control samples. (C) KEGG pathway analysis of DEGs at each stage in G28 compared with CBH. Changes in p-value are indicated by a change in color. The darker color is used to represent the smaller p-value.
Figure 6
Figure 6
Heatmap analysis of genes associated with (A) ABA, (B) BR, (C) CK, (D) IAA, (E) ETH, (F) GA, (G) SA, and (H) JA signal transduction after infection of G28 and CBH tobacco varieties with PVY MSNR. Changes in expression level are indicated by a change in color. Red is used to represent the up-regulation of the genes, and blue is used to represent the down-regulation of the genes.
Figure 7
Figure 7
Heatmap analysis of genes associated with starch and sucrose metabolism after infection of G28 and CBH tobacco varieties with PVY MSNR. Changes in expression level are indicated by a change in color. Red is used to represent the up-regulation of the genes, and blue is used to represent the down-regulation of the genes.
Figure 8
Figure 8
Heatmap analysis of genes associated with phenylpropanoid biosynthesis after infection of G28 and CBH tobacco varieties with PVY MSNR. Changes in expression level are indicated by a change in color. Red is used to represent the up-regulation of the genes, and blue is used to represent the down-regulation of the genes.
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