Mechanism of conjugation. II. Characterization of an Hfr dna ts mutant of Escherichia coli K-12
- PMID: 377019
- DOI: 10.1007/BF00276221
Mechanism of conjugation. II. Characterization of an Hfr dna ts mutant of Escherichia coli K-12
Abstract
A new conditional thermosensitive Hfr mutant of Escherichia coli K-12 was isolated. The ts mutation is cotransducible with purE and tsx loci on the E. coli chromosome. Upon temperature shift to 42 degrees C the DNA synthesis and transfer of chromosome is stopped immediately and RNA, protein synthesis in about ten minutes.
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