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. 1979 Apr 17;172(1):107-11.
doi: 10.1007/BF00276221.

Mechanism of conjugation. II. Characterization of an Hfr dna ts mutant of Escherichia coli K-12

A Blinkowa, G Gawecka

Mechanism of conjugation. II. Characterization of an Hfr dna ts mutant of Escherichia coli K-12

A Blinkowa et al. Mol Gen Genet. .

Abstract

A new conditional thermosensitive Hfr mutant of Escherichia coli K-12 was isolated. The ts mutation is cotransducible with purE and tsx loci on the E. coli chromosome. Upon temperature shift to 42 degrees C the DNA synthesis and transfer of chromosome is stopped immediately and RNA, protein synthesis in about ten minutes.

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