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Multicenter Study
. 2023 Oct;42(10):1245-1250.
doi: 10.1007/s10096-023-04656-z. Epub 2023 Sep 13.

A LAMP-based assay for the molecular detection of group B Streptococcus

Coralie Lemaire  1 , Mélinda Cheminet  2 , Claire Duployez  3 , Mathilde Artus  4 , Yassine Ballaa  2 , Laura Devos  3 , Céline Plainvert  2   5 , Claire Poyart  2   5 , Florence Le Gall  4 , Asmaa Tazi  2   5 , Philippe Lanotte  6
Affiliations
Multicenter Study

A LAMP-based assay for the molecular detection of group B Streptococcus

Coralie Lemaire et al. Eur J Clin Microbiol Infect Dis. 2023 Oct.

Abstract

Purpose: Streptococcus agalactiae remains a major pathogen in human health, especially in neonatal infection. Detection in pregnant women is essential to initiate intrapartum antibiotic prophylaxis. This study compared the HiberGene loop-mediated isothermal amplification (LAMP) assay to culture, the reference method, for the detection of group B Streptococcus (GBS) in pregnant women.

Methods: This was a prospective multicenter study conducted in four French hospitals. Three hundred fifty-four non-redundant routine care vaginal swabs were analyzed by both methods, LAMP assay and culture. Clinicians and patients were blinded to the results of the LAMP assay.

Results: Three hundred thirty-seven samples presented concordant results, 15 presented discordant results, and 2 were invalid using the LAMP assay (excluded from the study). Compared to culture, the LAMP assay had a sensitivity of 87.7%, a specificity of 98%, a negative predictive value of 97.6%, and a positive predictive value of 89.3%.

Conclusion: The HiberGene GBS LAMP assay is an easy test that possesses good performances compared with the reference method, culture. It could be used in case of emergency when a quick result is needed.

Keywords: LAMP; Neonatal infection; Pregnant; Screening; Streptococcus agalactiae.

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