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. 2023 Sep 13;16(1):216.
doi: 10.1186/s13104-023-06506-9.

Bacterial endotoxin lipopolysaccharides regulate gene expression in human colon cancer cells

Heping Cao  1
Affiliations

Bacterial endotoxin lipopolysaccharides regulate gene expression in human colon cancer cells

Heping Cao. BMC Res Notes. .

Abstract

Objective: Lipopolysaccharide (LPS) is a major cell wall component of gram-negative bacteria. Colon bacteria contribute to LPS which promotes colon cancer metastasis. The objective of this study was to survey the effect of LPS on cell viability and gene expression of 55 molecular targets in human colon cancer cells.

Results: LPS did not affect the viability of COLO 225 cells under the culture conditions but affected the expression of a number of genes important in inflammatory responses and cancer development. LPS increased TTP family, GLUT family and DGAT1 mRNA levels but decreased DGAT2a and DGAT2b expression in the human colon cancer cells. LPS also increased COX2, CXCL1, ELK1, ICAM1, TNFSF10 and ZFAND5 but decreased BCL2L1, CYP19A1 and E2F1 mRNA levels in the colon cancer cells. These data suggest that LPS has profound effects on gene expression in human colon cancer cells.

Keywords: Colon cancer cell; Cytotoxicity; Gene expression; Lipopolysaccharide.

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Conflict of interest statement

The author declares no competing interests.

Figures

Fig. 1
Fig. 1
Effect of LPS on the expression of gossypol-regulated genes as well as DGAT and GLUT family genes
Fig. 2
Fig. 2
Effect of LPS on TTP and IL, proinflammatory and other family gene expression
See this image and copyright information in PMC

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