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. 2023 Nov;29(11):2380-2382.
doi: 10.3201/eid2911.230836. Epub 2023 Sep 13.

Genomic Sequencing Surveillance to Identify Respiratory Syncytial Virus Mutations, Arizona, USA

LaRinda A Holland, Steven C Holland, Matthew F Smith, Victoria R Leonard, Vel Murugan, Lora Nordstrom, Mary Mulrow, Raquel Salgado, Michael White, Efrem S Lim

Genomic Sequencing Surveillance to Identify Respiratory Syncytial Virus Mutations, Arizona, USA

LaRinda A Holland et al. Emerg Infect Dis. 2023 Nov.

Abstract

We conducted surveillance of respiratory syncytial virus (RSV) genomic sequences for 100 RSV-A and 27 RSV-B specimens collected during November 2022-April 2023 in Arizona, USA. We identified mutations within prefusion F-protein antigenic sites in both subtypes. Continued genomic surveillance will be critical to ensure RSV vaccine effectiveness.

Keywords: Arizona; RSV; United States; antigenic sites; genomic surveillance; respiratory infections; respiratory syncytial virus; viruses.

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Figures

Figure
Figure
Genomic sequencing analysis of RSV in Arizona, USA, 2022–2023. A) Five-week moving average of PCR-confirmed RSV detections in Arizona reported to the National Respiratory and Enteric Virus Surveillance System and RSV sequence counts by genotype obtained for specimens used in this study. B) Relative abundance of RSV-A and RSV-B genotypes shown for all RSV genomes (RSV-A, n = 1,047; RSV-B, n = 941) deposited in GISAID (https://www.gisaid.org) with collection dates January 1, 2022–May 1, 2023, including genotypes obtained for specimens used in this study (RSV-A n = 100; RSV-B, n = 27). C) Reverse transcription PCR Ct values and genome coverage for RSV-A and RSV-B samples. Ct, cycle threshold; RSV, respiratory syncytial virus.
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