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. 2023 Aug 28:2023:10.17912/micropub.biology.000921.
doi: 10.17912/micropub.biology.000921. eCollection 2023.

Antimicrobial activity of Paenarthrobacter nicotinovorans

Justyna Kakol  1 , Mainor Vang  1 , Drew Sausen  1 , Tracey Steeno  1 , Angelo Kolokithas  1
Affiliations

Antimicrobial activity of Paenarthrobacter nicotinovorans

Justyna Kakol et al. MicroPubl Biol. .

Abstract

Antibiotic resistance is one of the biggest global challenges of the century. Many pathogens have become resistant to antibiotics due to human misuse and effective ones are in short supply. Discovering potential new antibiotic molecules may help to address the current antibiotic shortage. To this end, the Tiny Earth program, which engages students from around the world to discover potential antibiotic producing microbes from the local soil, was utilized. Students collect, screen, and isolate bacteria from their local communities in hopes of addressing the global crisis of antibiotic resistance. One such microbe, Paenarthrobacter nicotinovorans , was isolated and screened for antimicrobial activity.

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Conflict of interest statement

The authors declare that there are no conflicts of interest present.

Figures

Figure 1. Isolation and screening of antimicrobial activity in MV2 bacteria
Figure 1. Isolation and screening of antimicrobial activity in MV2 bacteria
A. 20 bacterial colonies were patched onto an LB plate from soil sample. B. The 20 isolated bacteria from Figure 1 A. were patched on top of Erwinia carotovra and activity (clearing) was checked after 24 hours later. C. Sample in spot 2 (MV2) from Figure 1A was patched on top of the following pathogens: (1) Staphylococcus epidermidis ; (2) Pseudomonas putida ; (3) Enterobacter aerogenes ; (4) Mycobacterium smegmatis ; (5) Bacillus subtilis ; (6) Acinetobacter bacilli; (7) Erwinia carotovora ; (8) Escherichia coli. Activity was checked 24 hours later. Testing isolated secondary metabolites against two strains of ESKAPE pathogens: D. Staphylococcus epiermidis and E. Erwinia carotovora by using spot-plate method. Numbers correspond to the following isolates. 1. SEC 10 – organic layer of secondary metabolite , 2. BB12- organic layer of secondary metabolite, 3. CM19 - organic layer of secondary metabolite, 4. MV2- organic layer of secondary metabolite with small amount of aqueous layer 5. BS16 - organic layer of secondary metabolite with small amount of aqueous layer 6. MV2 - organic layer of secondary metabolite 7. BS16 – organic layer of secondary metabolite 8. Negative control 9. Positive control.
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