Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Sep 16;39(11):310.
doi: 10.1007/s11274-023-03747-5.

Evaluating β-cryptoxanthin antioxidant properties against ROS-induced macromolecular damages and determining its photo-stability and in-vitro SPF

Daiji Brahma  1 , Debjani Dutta  2
Affiliations

Evaluating β-cryptoxanthin antioxidant properties against ROS-induced macromolecular damages and determining its photo-stability and in-vitro SPF

Daiji Brahma et al. World J Microbiol Biotechnol. .

Abstract

Natural antioxidants have become vital to minimize macromolecular damage caused by Reactive Oxygen Species (ROS). This study investigated the antioxidant property of β-cryptoxanthin (β-CRX) extracted from Kocuria marina DAGII and its protective effect against macromolecular damages by generating ROS via two models: UV radiation and the Fenton reaction. β-cryptoxanthin exhibited the highest scavenging activity towards hydrogen peroxide radicals with an IC50 value of 38.30 ± 1.13 μg/ml, favoring the hydrogen atom transfer mechanism. The total antioxidant capacity value of 872.0101 ± 1.84 μg BHT/mg β-CRX indicated the cumulative ROS scavenging ability of β-cryptoxanthin. β-cryptoxanthin could protect against ROS-induced lipid peroxidation, protein oxidation, and DNA damage. The highest lipid peroxidation and protein oxidation inhibition values of β-cryptoxanthin against ROS were 99.371 ± 0.51% and 78.19 ± 0.15%, respectively. β-cryptoxanthin also showed a protective effect in maintaining DNA intactness against ROS-mediated DNA damage. Allium cepa test showed the non-genotoxic nature of β-cryptoxanthin and its protective effect against ROS genotoxic effects. A photo-stability study of β-cryptoxanthin toward UVA and UVB radiation showed a rapid bleaching result of UVB obeying pseudo-zero order kinetics with an average R2 value of 0.9897 and a higher k value (-6.3 × 10-11 ± 0.2 M/s) than UVA (k value -3.1 × 10-11 ± 0.17 M/s), signifying that UVB is more potent toward photo-degradation. The good SPF value of 23.1737 ± 0.15 showed the UV protection capability of β-cryptoxanthin. Thus, the present study suggests that β-cryptoxanthin could be a valuable antioxidant to protect against ROS-induced various macromolecular damages and act as a good UV protectant.

Keywords: Antioxidant; Genotoxicity; Kocuria marina sp; Photo-protection; Reactive oxygen species.

PubMed Disclaimer

References

    1. Aissous I, Benrebai M, Cacan E, Berkel C, Erenler R, Souad A, Samir B, Benayache F, Chawki B (2021) Antioxidant and antiproliferative activities of the n-butanol extract of Centaurea maroccana Ball aerial parts. Curr Issues Pharm Med Sci 34:5–11. https://doi.org/10.2478/cipms-2021-0002 - DOI
    1. Alabi OA, Atanda HC, Olumurewa JAV (2022) Cytogenotoxicity of the aqueous extract of Parquetina nigrescens leaf using Allium cepa assay. Protoplasma 259:1417–1425. https://doi.org/10.1007/s00709-022-01741-6 - DOI - PubMed
    1. Ali ZS, Muhammad D, Zrieki A (2022) In vitro Assessment of sun protection factor (SPF) and Antioxidant activity of Viola odorata extracts. Res J Pharmacy Technol 15(2):655–660
    1. Ansary TM, Hossain MR, Kamiya K, Komine M, Ohtsuki M (2021) Inflammatory molecules associated with ultraviolet radiation-mediated skin aging. Int J Mol Sci 22(8):3974. https://doi.org/10.3390/ijms22083974 - DOI - PubMed - PMC
    1. Awah FM, Verla AW (2010) Antioxidant activity, nitric oxide scavenging activity and phenolic contents of Ocimum gratissimum leaf extract. J Med Plants Res 4(24):2479–2487. https://doi.org/10.5897/JMPR10.407 - DOI

LinkOut - more resources