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Review
. 2024 Mar;30(3):e3545.
doi: 10.1002/psc.3545. Epub 2023 Sep 18.

Recent progress in the reprogramming of nonribosomal peptide synthetases

Fumihiro Ishikawa  1 , Shinya Nakamura  1 , Isao Nakanishi  1 , Genzoh Tanabe  1
Affiliations
Review

Recent progress in the reprogramming of nonribosomal peptide synthetases

Fumihiro Ishikawa et al. J Pept Sci. 2024 Mar.

Abstract

Nonribosomal peptide synthetases (NRPSs) biosynthesize nonribosomal peptide (NRP) natural products, which belong to the most promising resources for drug discovery and development because of their wide range of therapeutic applications. The results of genetic, biochemical, and bioinformatics analyses have enhanced our understanding of the mechanisms of the NRPS machinery. A major goal in NRP biosynthesis is to reprogram the NRPS machinery to enable the biosynthetic production of designed peptides. Reprogramming strategies for the NRPS machinery have progressed considerably in recent years, thereby increasing the yields and generating modified peptides. Here, the recent progress in NRPS reprogramming and its application in peptide synthesis are described.

Keywords: adenylation domain; natural product biosynthesis; nonribosomal peptide synthetase; peptide natural products; reprogramming.

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