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Comparative Study
. 1979 Apr;6(4):1571-81.
doi: 10.1093/nar/6.4.1571.

Role of ribothymidine in the thermal stability of transfer RNA as monitored by proton magnetic resonance

P Davanloo, M Sprinzl, K Watanabe, M Albani, H Kersten
Free PMC article
Comparative Study

Role of ribothymidine in the thermal stability of transfer RNA as monitored by proton magnetic resonance

P Davanloo et al. Nucleic Acids Res. 1979 Apr.
Free PMC article

Abstract

In order to elucidate the functional role of the modified uridines at position 54 of tRNA, the 270 MHz high-field proton NMR spectra of methionine tRNAs from E. coli, from a mutant thereof, and from T. thermophilus, containing ribothymidine, uridine and 2-thioribothymidine, respectively, have been measured as a function of temperature. A comparison of the NMR melting profiles of the minor nucleosides from these tRNAs shows that the melting temperature of uridine containing tRNA is 6 degrees C lower than that of the wild type tRNA whereas that of the 2-thioribothymidine tRNA is 7 degrees C higher than that of the wild type tRNA. These results, therefore, demonstrate that these modifications serve for stabilization of the tertiary structure of tRNA.

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References

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