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. 2023 Sep 20;190(10):402.
doi: 10.1007/s00604-023-05994-7.

Sulfonic acid functionalized monolithic column for high selectivity capillary electrochromatography separation

Shuyu Dai #  1 , Yun Guo #  1 , Hongyan Mao  1 , Xiaohui Wei  1 , Wenfen Zhang  2   3 , Xin Chen  1 , Wuduo Zhao  1 , Shusheng Zhang  4   5
Affiliations

Sulfonic acid functionalized monolithic column for high selectivity capillary electrochromatography separation

Shuyu Dai et al. Mikrochim Acta. .

Abstract

A new nano-scale spherical vinyl-functionalized covalent organic polymer (TAPT-DVA-COP) with uniform sizes around 300 nm was initially constructed using 2,5-divinyl-1,4-benzaldehyde (DVA) and 2,4,6-tris(4-aminophenyl)-1,3,5-triazine (TAPT) as monomers. Then, a sulfonic acid (-SO3H) modified COP termed COP-SO3H was developed based on post-sythesis method employing TAPT-DVA-COP as precursor. Capillary electrochromatography (CEC) monolithic columns were fabricated using the physical doping technique to exhibit the application potential of TAPT-DVA-COP and COP-SO3H. Compared to the TAPT-DVA-COP monolithic column, the COP-SO3H monolithic column achieved a highly selective separation between analytes with different properties, including monosubstituted benzenes, alkylbenzenes, hydroxybenzoates, nucleoside bases, and biogenic amines. Non-covalent interaction (NCI) analysis and experimental data show that the synergism of the sulfonic acid group and aromatic moieties on COP-SO3H endows the new stationary phase with diverse interactions, including ion exchange, hydrophobic, π-π and hydrogen bonding. In addition, the COP-SO3H monolithic column exhibited good reproducibility and excellent potential for the determination of hydroxybenzoates in compact powders and alkylbenzenes in effluent samples.

Keywords: Capillary electrochromatography; Monolithic capillary; Nano spherical covalent organic polymers; Sulfonic acid functionalization.

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