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. 2023 Oct 19;12(10):e0052723.
doi: 10.1128/MRA.00527-23. Epub 2023 Sep 20.

Nanopore PCR-cDNA sequencing of the biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) informs gene annotation

Andrew Wesley Legan  1 , Hillary L Mehl  1 , Alex Varaksa  1 , Kenneth A Callicott  1
Affiliations

Nanopore PCR-cDNA sequencing of the biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) informs gene annotation

Andrew Wesley Legan et al. Microbiol Resour Announc. .

Abstract

Toxic molds in the Aspergillus genus synthesize carcinogenic aflatoxins which contaminate crops. The widely applied biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) has a high-quality public genome but lacks corresponding gene annotations. We generated high-quality gene predictions for this isolate by using long-read Nanopore PCR-cDNA sequencing.

Keywords: Aspergillus; Nanopore; aflatoxin; annotation; transcription.

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Conflict of interest statement

The authors declare no conflict of interest.

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