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. 2023 Nov;116(11):1209-1225.
doi: 10.1007/s10482-023-01877-2. Epub 2023 Sep 22.

Stieleria tagensis sp. nov., a novel member of the phylum Planctomycetota isolated from Tagus River in Portugal

Ofélia Godinho  1   2 , Dominika Klimek  3   4 , Adrianna Jackiewicz  5 , Bárbara Guedes  5 , Eduarda Almeida  5   6 , Rita Calisto  5   6 , Inês Rosado Vitorino  5   6 , José Diogo Neves Santos  5   6 , Ignacio González  7 , Alexandre Lobo-da-Cunha  8 , Magdalena Calusinska  3 , Sandra Quinteira  5   9   10 , Olga Maria Lage  5   6
Affiliations

Stieleria tagensis sp. nov., a novel member of the phylum Planctomycetota isolated from Tagus River in Portugal

Ofélia Godinho et al. Antonie Van Leeuwenhoek. 2023 Nov.

Abstract

A bacterial strain was isolated from a brackish water sample of Tagus river, Alcochete, Portugal and was designated TO1_6T. It forms light pink colonies on M13 medium supplemented with N-acetylglucosamine. Cells are pear-shaped to spherical, form rosettes and divide by budding. Strain TO1_6T presents a mesophilic and neutrophilic profile, with optimum growth at 20 to 25 °C and pH 7.0 to 7.5, and vitamin supplementation is not required to promote its growth. The genome of the novel isolate is 7.77 Mbp in size and has a DNA G + C content of 56.3%. Based on its 16S rRNA gene sequence, this strain is affiliated with the phylum Planctomycetota. Further taxonomic characterization using additional phylogenetic markers, namely rpoB gene sequence (encoding the β-subunit of the DNA-dependent RNA polymerase), as well as Percentage of conserved proteins, average nucleotide identity and average amino acid identity, suggest the affiliation of strain TO1_6T to the genus Stieleria, a recently described taxon in the family Pirellulaceae, order Pirellulales and class Planctomycetia. Based on the genotypic, phylogenetic and physiological characterization, we here describe a new species represented by the type strain TO1_6T (= CECT 30432T, = LMG 32465T), for which the name Stieleria tagensis sp. nov. is proposed.

Keywords: Brackish water; Genomic identification; Mesophilic aerobic bacterium; Pirellulaceae; Planctomycetota.

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Conflict of interest statement

The authors declare no competing interests.

The authors declare that there are no conflicts of interest.

Figures

Fig. 1
Fig. 1
Phylogenetic 16S rRNA gene sequence-based dendrogram demonstrating the relationship between the type species of the different genera within the families Lacipirellulaceae, Thermoguttaceae and Pirellulaceae and the described species of the genus Stieleria, including the new strain TO1_6T. Phycisphaera mikurensis was used as outgroup. The numbers on the branches refer to percentage of trees in which the associated taxa clustered together from the total of bootstrap replications. GenBank accession numbers are presented. The scale bar refers to 0.02 substitutions per nucleotide position
Fig. 2
Fig. 2
Multi-locus sequence analysis-based phylogenetic tree highlighting the position of the novel strain TO1_6T. The web-based tool autoMLST was used to construct the phylogenetic tree, with 1000 bootstrap (values shown in % at the nodes). RefSeq accession numbers are presented. Genomes from Verrucomicrobium spinosum DSM 4136T and Phycisphaera mikurensis NBRC 102666T were used as outgroups. Scale bar represents 0.10 substitutions per nucleotide position
Fig. 3
Fig. 3
TEM images of strain TO1_6T depicting its ultrastructure. R—inclusion storage bodies; asterisk—Condensed DNA; OM—outer membrane; CM—cytoplasmic membrane; arrow head—invaginations of the cytoplasmic membrane; arrow—fimbriae; B—bud
See this image and copyright information in PMC

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