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. 2024 Jan 15:267:125226.
doi: 10.1016/j.talanta.2023.125226. Epub 2023 Sep 20.

Multielement analysis of single red blood cells by single cell - inductively coupled plasma tandem mass spectrometry

Mathieu Melczer  1 , Javier Jiménez Lamana  2 , Ana Justo-Vega  3 , Ogier Hanser  4 , Sophie Ndaw  4 , Ryszard Lobinski  5
Affiliations

Multielement analysis of single red blood cells by single cell - inductively coupled plasma tandem mass spectrometry

Mathieu Melczer et al. Talanta. .

Abstract

A method for the analysis of essential metals (Fe, Cu, Mg, and Zn) and non-metals (P, S) in single red blood cells was developed by single cell (SC)-ICP-MS. The use of a triple quadrupole configuration (MS/MS) enabled an effective elimination of polyatomic interferences, which affect the accuracy of ICP-MS analysis using a single quadrupole mass analyzer. Fixation with glutaraldehyde for at least 90 days was developed to improve the quantification of elements in a single red blood cell. The experimental conditions were optimized while special attention was paid to the residence time of analytes in the plasma. Addition of a surfactant (0.05% (v/v) Tween80®) improved quantification of elements in fixed red blood cells. The detection limits obtained by SC-ICP-MS/MS were lower than for ICP-MS, especially for S and P (3 fg and 1.7 fg. cell-1 instead of 163 and 6.3 fg. cell-1, respectively).

Keywords: Erythrocytes; Fixation; ICP-MS/MS; Red blood cells; SC-ICP-MS; Single cell.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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