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. 2023 Sep 7:14:1236906.
doi: 10.3389/fmicb.2023.1236906. eCollection 2023.

Micromonospora profundi TRM 95458 converts glycerol to a new osmotic compound

Di Lu  1   2 , Hong-Ling Shen  1   2 , Lei Wang  1   2 , Chuan-Xing Wan  1   2
Affiliations

Micromonospora profundi TRM 95458 converts glycerol to a new osmotic compound

Di Lu et al. Front Microbiol. .

Abstract

Plant growth and agricultural productivity was greatly limited by soil salinity and alkalization. The application of salt-tolerant rhizobacteria could effectively improve plant tolerance to saline-alkali stress. Micromonospora profundi TRM 95458 was obtained from the rhizosphere of chickpea (Cicer arietinum L.) as a moderate salt-tolerant rhizobacteria. A new osmotic compound (ABAGG) was isolated from the fermentation broth of M. profundi TRM 95458. The chemical structure of the new compound was elucidated by analyzing nuclear magnetic resonance (NMR) and high-resolution mass (HRMS) data. M. profundi TRM 95458 could convert glycerol into ABAGG. The accumulation of ABAGG varied depending on the amount of glycerol and glycine added to the fermentation medium. In addition, the concentration of NaCl affected the ABAGG content obviously. The highest yield of ABAGG was observed when the salt content of the fermentation medium was 10 g/L. The study indicated that salt stress led to the accumulation of ABAGG using glycerol and glycine as substrates, suggesting ABAGG might aid in the survival and adaptation of the strain in saline-alkaline environments as a new osmotic compound.

Keywords: ABAGG; Micromonospora profundi TRM 95458; compatible solutes; conversion of glycerol; salt-tolerant rhizobacteria.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Colony characteristics and scanning electron micrograph of TRM95458 grown on ISP2 agar at 28°C for 7 days. (A) Colony characteristics of TRM95458. (B) The spores of TRM95458. (C) Neighbour-joining phylogenetic tree from the 16S rRNA sequences of TRM95458 and related species constructed by MEGA 5.05. Numbers at nodes indicate levels of bootstrap support (%) based on a neighbour-joining analysis of 1,000 resampled datasets; only values >50% are given. NCBI accession numbers are given. Bar, 0.01 nucleotide substitutions per site. Streptomyces olivaceous NRRL B-3009 was selected as the outgroup.
Figure 2
Figure 2
Structure of compounds 1, 2, 3,4.
Figure 3
Figure 3
pHPLC analysis of the crude extract of strain TRM95458 fermented in liquid fermentation medium (Glycerol 2%, peptone 1%, glycine 1%, yeast extract 1%), 80% methanol eluent was purified by preparative HPLC, Conditions: A: 30% MeOH-H2O (0.075% HCOOH); B: 100% MeOH; flow rate:10mL/min; gradient elution conditions: 0–40 min 0–65% B; 40–45 min 65–100%B; 45–50 min 100%B; DAD detector wavelength: 350 nm. 2, tR = 19.38 min; 1, tR = 21.85 min; 3, tR = 28.28 min; and 4, tR = 33.13 min. The crude extract was isolated by pHPLC equipped.
Figure 4
Figure 4
HRMS spectrum of compound 1 C12H15NaNO6+ (M+Na)+ 292.0791584.
Figure 5
Figure 5
1H NMR of compound 1 in MeOD.
Figure 6
Figure 6
13C NMR of compound 1 in MeOD.
Figure 7
Figure 7
HMBC correlations of compound 1.
Figure 8
Figure 8
The standard curve of ABAGG by HPLC.
Figure 9
Figure 9
Production of ABAGG by TRM 95458 with different glycerol addition.
Figure 10
Figure 10
Production of ABAGG by TRM 95458 with different glycine addition.
Figure 11
Figure 11
Relationship between salt stress and ABAGG accumulation in TRM 95458.
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