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. 2023 Aug 31;19(4):82.
doi: 10.3892/mco.2023.2678. eCollection 2023 Oct.

Genetic analysis of fundic gland‑type gastric adenocarcinoma

Lei Liu  1 , Xuedong Zhang  2 , Xue Fan  3 , Xiaoyun Zhu  1
Affiliations

Genetic analysis of fundic gland‑type gastric adenocarcinoma

Lei Liu et al. Mol Clin Oncol. .

Abstract

This study aimed to analyze the molecular characteristics of gastric adenocarcinoma of the fundic-gland type (GAFG) and explore the possible mechanism of tumor development. Samples from 10 Chinese patients with GAFG were collected at the Peking University International Hospital and Liaocheng People's Hospital between January 2015 and March 2022. The nucleic acid sequence of Epstein Barr virus-encoded RNA (EBV-EBER) was detected by in situ hybridization. Genetic mutation information for GNAS, KRAS, NRAS, BRAF, PIK3CA, TP53, APC, CTNNB1, HER2, MLH1, MSH2, MSH6, and PMS2 was obtained by Next-Generation Sequencing, and the relevant literature was reviewed. A total of eight instances of missense mutations were detected, consisting of seven cases with GNAS mutations, two cases with KRAS mutations, and one case with a TP53 mutation. Additionally, two patients had simultaneous missense mutations in GNAS and KRAS. Nonsynonymous mutations in APC, CTNNB1, NRAS, BRAF, PIK3CA, HER2, MLH1, MSH2, MSH6, or PMS2 were not observed in any cases. In addition, all tumors were EBER-negative. GAFG exhibits diversity at the molecular level, and GNAS mutations are more common than KRAS mutations, TP53 mutations, and microsatellite instability. To date, no association between EBV/HER2 and GAFG has been found.

Keywords: Epstein Barr virus; GNAS; HER2; KRAS; fundus gland; gastric neoplasm.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
The Epstein Barr virus-encoded RNA status of gastric adenocarcinoma of the fundic-gland type was negative as determined by in situ hybridization. The nuclei were stained blue with hematoxylin. Magnification, x40.
Figure 2
Figure 2
Positive control of Epstein Barr virus-encoded RNA from non-keratinizing nasopharyngeal carcinoma. The nuclei were stained brown with DAB. Magnification, x100.
Figure 3
Figure 3
GNAS missense mutation (case #9). The 601st site in the gene sequence was mutated from a cytosine to a thymine.
Figure 4
Figure 4
KRAS missense mutation (case #9). The 179th site in the gene sequence was mutated from a guanine to adenine.
See this image and copyright information in PMC

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