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. 1986 Oct 10;14(19):7661-74.
doi: 10.1093/nar/14.19.7661.

Hybridization triggered cross-linking of deoxyoligonucleotides

T R Webb, M D Matteucci
Free PMC article

Hybridization triggered cross-linking of deoxyoligonucleotides

T R Webb et al. Nucleic Acids Res. .
Free PMC article

Abstract

This paper reports details of the synthesis of oligodeoxynucleotides containing the modified base 5-methyl-N4,N4-ethanocytosine (Ce). The 9-fluorenylmethoxycarbonyl group is used as a protecting group for the exocyclic amines of dA and dC. This group can be removed rapidly under very mild conditions. Oligomers containing the Ce base form a cross-link when hybridized to their complementary deoxyoligonucleotides. Some of the scope and limitations of these cross-link forming oligonucleotides are reported.

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References

    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. J Mol Biol. 1978 Jun 25;122(2):145-62 - PubMed
    1. Nature. 1983 Jan 20;301(5897):214-21 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Mar;83(5):1227-31 - PubMed
    1. Nucleic Acids Res. 1985 Nov 25;13(22):8181-96 - PubMed

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