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. 2023 Sep 21;18(1):20230787.
doi: 10.1515/med-2023-0787. eCollection 2023.

Pepsin concentration in oral lavage fluid of rabbit reflux model constructed by dilating the lower esophageal sphincter

Zhezhe Sun  1 , Wei Wu  1 , Gang Wang  1 , Lianyong Li  2 , Lei Wang  1 , Hongdan Liu  1
Affiliations

Pepsin concentration in oral lavage fluid of rabbit reflux model constructed by dilating the lower esophageal sphincter

Zhezhe Sun et al. Open Med (Wars). .

Abstract

The aim of this study was to explore the changes in pH and pepsin concentrations in oral lavage fluid of rabbit reflux model. A total of 18 New Zealand rabbits were randomly divided into two groups. The lower esophageal sphincters (LESs) of the rabbits in the experimental group (EG) were dilated by balloon after the LESs were localized by manometry. The pH levels of the throat and the lower esophagus were monitored 1 week before and 2 weeks after inflation. Oral lavage fluid was collected 1 week before, and 2 and 8 weeks after inflation. The pH monitoring showed that the percentage of reflux time, the number of reflux events, and the longest time of reflux after the dilation (AE) in the EG were significantly higher than before the dilation (P < 0.01). The pepsin concentrations at 2 and 8 weeks AE in the EG were significantly higher than that before and that in the control group (P < 0.05). Based on receiver operating characteristic curve analysis, the best diagnostic threshold value was 30.3 ng/ml. The reflux model constructed by balloon inflation of the LES in rabbits is characterized by a decrease in throat pH and an increase in salivary pepsin concentration.

Keywords: animal model; balloon dilation; gastroesophageal reflux; laryngopharyngeal reflux.

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Conflict of interest statement

Conflict of interest: All authors declare that they have no conflict of interest.

Figures

Figure 1
Figure 1
pH monitoring data BE and AE of animal 2 in the EG. (a) No reflux event was found in throat pH monitoring 1 week BE in EG dilation. (b) Acid reflux event with pH < 5 was found in throat pH monitoring 2 weeks AE in EG.
Figure 2
Figure 2
Pepsin concentrations in oral lavage fluid 1 week BE, and at 2 and 8 weeks AE in the experimental animals. *P < 0.05.
Figure 3
Figure 3
ROC for diagnosis of LPRD based on pepsin concentration in rabbit oral lavage fluid.
See this image and copyright information in PMC

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