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Review
. 2023 Sep 12;13(9):882.
doi: 10.3390/bios13090882.

Circulating Tumor Cells Adhesion: Application in Biosensors

Eduarda B Paglia  1 , Estela K K Baldin  1   2 , Gabriela P Freitas  1   2 , Thalyta S A Santiago  1 , João B M R Neto  3 , Jorge V L Silva  2 , Hernandes F Carvalho  4 , Marisa M Beppu  1
Affiliations
Review

Circulating Tumor Cells Adhesion: Application in Biosensors

Eduarda B Paglia et al. Biosensors (Basel). .

Abstract

The early and non-invasive diagnosis of tumor diseases has been widely investigated by the scientific community focusing on the development of sensors/biomarkers that act as a way of recognizing the adhesion of circulating tumor cells (CTCs). As a challenge in this area, strategies for CTCs capture and enrichment currently require improvements in the sensors/biomarker's selectivity. This can be achieved by understanding the biological recognition factors for different cancer cell lines and also by understanding the interaction between surface parameters and the affinity between macromolecules and the cell surface. To overcome some of these concerns, electrochemical sensors have been used as precise, fast-response, and low-cost transduction platforms for application in cytosensors. Additionally, distinct materials, geometries, and technologies have been investigated to improve the sensitivity and specificity properties of the support electrode that will transform biochemical events into electrical signals. This review identifies novel approaches regarding the application of different specific biomarkers (CD44, Integrins, and EpCAm) for capturing CTCs. These biomarkers can be applied in electrochemical biosensors as a cytodetection strategy for diagnosis of cancerous diseases.

Keywords: CD44; EpCAm; circulating tumor cells; electrochemical biosensor; integrins.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Number of publications related to the topic of this article in the last 10 years, using Scopus as a database (keywords “electrochemical sensor” and “tumoral cells”).
Figure 2
Figure 2
A potential approach involves creating label-free electrochemical biosensors for cancer detection. This entails enhancing electrodes with nanotechnology-based materials, immobilizing biorecognition elements using specific protocols, and utilizing robust electrochemical detection methods [26].
Figure 3
Figure 3
Biorecognition strategies for application in electrochemical biosensors used for adhesion of circulating tumor cells.
See this image and copyright information in PMC

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