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. 2023 Nov;415(27):6701-6709.
doi: 10.1007/s00216-023-04947-6. Epub 2023 Sep 27.

Plastic probe electrospray ionization mass spectrometry developed for rapid fingerprint profile of biological samples without pretreatment

Shuanglong Wang  1   2   3 , Shan Shan  4 , Jian Yang  3   5 , Junhui Zhou  2 , Guofeng Wang  6
Affiliations

Plastic probe electrospray ionization mass spectrometry developed for rapid fingerprint profile of biological samples without pretreatment

Shuanglong Wang et al. Anal Bioanal Chem. 2023 Nov.

Abstract

A triangular-shaped flat plastic substrate probe was prepared for direct electrospray ionization mass spectrometry (ESI-MS) for analysis of untreated chemical and biological samples including liquids (Met-Arg-Phe-Ala peptide, reserpine, and dodecyl aldehyde), solids (biological samples, traditional Chinese medicine), and powders (roasted coffee, rhizoma coptidis, lotus plumule, and Schisandra sphenanthera). Quantitative analysis of reserpine in water yielded a detection limit of 1 ng mL-1, dynamic response range within 1-500 ng mL-1, and linearity of signal response ˃0.9925. Compared to the conventional capillary ESI, this plastic probe ESI offers lower cost of analysis (US $0.0056 per probe), higher sensitivity, lower sample consumption, longer signal duration (>6 min), better reproducibility, signal stability, and higher speed of analysis (<10 s per sample, including sample loading). Overall, the results indicate the potential of ESI-MS based on flat plastic probes as a versatile method for simple, sensitive, and stable analysis of untreated biological sample analysis.

Keywords: Direct analysis; Electrospray ionization (ESI); Mass spectrometry; Plastic probe; Rapid screening.

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