Exploring the Utility of Multiplex Infectious Disease Panel Testing for Diagnosis of Infection in Different Body Sites: A Joint Report of the Association for Molecular Pathology, American Society for Microbiology, Infectious Diseases Society of America, and Pan American Society for Clinical Virology

doi:10.1016/j.jmoldx.2023.08.005

Review

. 2023 Dec;25(12):857-875.

doi: 10.1016/j.jmoldx.2023.08.005. Epub 2023 Sep 26.

Exploring the Utility of Multiplex Infectious Disease Panel Testing for Diagnosis of Infection in Different Body Sites: A Joint Report of the Association for Molecular Pathology, American Society for Microbiology, Infectious Diseases Society of America, and Pan American Society for Clinical Virology

Affiliations

¹ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Roche Molecular Systems, San Clemente, California. Electronic address: michael.lewinski@roche.com.
² Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Department of Pathology and Laboratory Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina.
³ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Clinical Microbiology Service, Departments of Laboratory Medicine and Medicine, Memorial Sloan Kettering Cancer Center, New York, New York.
⁴ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Department of Clinical Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California.
⁵ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Department of Pathology and Laboratory Medicine, Children's Hospital Los Angeles and Keck School of Medicine, University of Southern California, Los Angeles, California.
⁶ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington.
⁷ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; University of Utah School of Medicine and ARUP Laboratories, Salt Lake City, Utah.
⁸ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Department of Microbiology, LabCorp Seattle, Seattle, Washington.
⁹ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Bio-Rad Laboratories, Irvine, California.
¹⁰ Association for Molecular Pathology, Rockville, Maryland.
¹¹ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, South Carolina.

PMID: 37757952
PMCID: PMC11702286
DOI: 10.1016/j.jmoldx.2023.08.005

Review

Exploring the Utility of Multiplex Infectious Disease Panel Testing for Diagnosis of Infection in Different Body Sites: A Joint Report of the Association for Molecular Pathology, American Society for Microbiology, Infectious Diseases Society of America, and Pan American Society for Clinical Virology

Michael A Lewinski et al. J Mol Diagn. 2023 Dec.

. 2023 Dec;25(12):857-875.

doi: 10.1016/j.jmoldx.2023.08.005. Epub 2023 Sep 26.

Authors

Affiliations

¹ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Roche Molecular Systems, San Clemente, California. Electronic address: michael.lewinski@roche.com.
² Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Department of Pathology and Laboratory Medicine, University of North Carolina School of Medicine, Chapel Hill, North Carolina.
³ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Clinical Microbiology Service, Departments of Laboratory Medicine and Medicine, Memorial Sloan Kettering Cancer Center, New York, New York.
⁴ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Department of Clinical Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California.
⁵ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Department of Pathology and Laboratory Medicine, Children's Hospital Los Angeles and Keck School of Medicine, University of Southern California, Los Angeles, California.
⁶ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington.
⁷ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; University of Utah School of Medicine and ARUP Laboratories, Salt Lake City, Utah.
⁸ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Department of Microbiology, LabCorp Seattle, Seattle, Washington.
⁹ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Bio-Rad Laboratories, Irvine, California.
¹⁰ Association for Molecular Pathology, Rockville, Maryland.
¹¹ Infectious Diseases Multiplex Working Group of the Clinical Practice Committee, Association for Molecular Pathology, Rockville, Maryland; Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, South Carolina.

PMID: 37757952
PMCID: PMC11702286
DOI: 10.1016/j.jmoldx.2023.08.005

Erratum in

Corrections.
[No authors listed] [No authors listed] J Mol Diagn. 2025 Mar;27(3):232. doi: 10.1016/j.jmoldx.2024.12.004. Epub 2025 Jan 16. J Mol Diagn. 2025. PMID: 39805327 Free PMC article. No abstract available.

Abstract

The use of clinical molecular diagnostic methods for detecting microbial pathogens continues to expand and, in some cases, supplant conventional identification methods in various scenarios. Analytical and clinical benefits of multiplex molecular panels for the detection of respiratory pathogens have been demonstrated in various studies. The use of these panels in managing different patient populations has been incorporated into clinical guidance documents. The Association for Molecular Pathology's Infectious Diseases Multiplex Working Group conducted a review of the current benefits and challenges to using multiplex PCR for the detection of pathogens from gastrointestinal tract, central nervous system, lower respiratory tract, and joint specimens. The Working Group also discusses future directions and novel approaches to detection of pathogens in alternate specimen types, and outlines challenges associated with implementation of these multiplex PCR panels.

PubMed Disclaimer

Conflict of interest statement

Disclosure Statement This article represents the consensus opinion of the Infectious Diseases Multiplex Working Group of the Clinical Practice Committee; all authors contributed equally. M.A.L. is employed by and owns stock options at Roche Molecular Systems. K.A. is employed by the University of North Carolina School of Medicine and has received honorarium from Cepheid. N.E.B. is employed by the Memorial Sloan Kettering Cancer Center and is a Clinical Advisory Board member at ArcBio and Scientific Advisory Board member at Bio-Rad Laboratories and Agena Bioscience. S.M.B.-W. is employed by the University of Southern California and has received consulting honoraria from Luminex and BioFire. J.D.B. is employed by the Children's Hospital of Los Angeles and the University of Southern California, is temporarily employed as a medical reviewer for GenMark Diagnostics, worked previously as a consultant and a member of the Expert Advisory panel for Accelerate Diagnostics, and is a current consultant for BioFire Diagnostics. A.L.G. is employed by the University of Washington Medical Center and is a recipient of research grants from Merck and Gilead and has central testing contracts with Abbott. S.N.N. is employed by and owns stock options at LabCorp Seattle. D.N. is employed by Bio-Rad Laboratories. F.N. is currently employed by Karius Inc. The remaining authors have declared no related conflicts of interests.

Cited by

Plasma Microbial Cell-Free DNA Sequencing from over 15,000 Patients Identified a Broad Spectrum of Pathogens.
Park SY, Chang EJ, Ledeboer N, Messacar K, Lindner MS, Venkatasubrahmanyam S, Wilber JC, Vaughn ML, Bercovici S, Perkins BA, Nolte FS. Park SY, et al. J Clin Microbiol. 2023 Aug 23;61(8):e0185522. doi: 10.1128/jcm.01855-22. Epub 2023 Jul 13. J Clin Microbiol. 2023. PMID: 37439686 Free PMC article.
Impact of Point-of-Care Testing on Diagnosis, Treatment, and Surveillance of Vaccine-Preventable Viral Infections.
Lakshmanan K, Liu BM. Lakshmanan K, et al. Diagnostics (Basel). 2025 Jan 7;15(2):123. doi: 10.3390/diagnostics15020123. Diagnostics (Basel). 2025. PMID: 39857007 Free PMC article. Review.
Viral meningitis in Sub-Saharan Africa: trends in prevalence, etiologies, and diagnostic approaches.
Geteneh A, Kiros M, Tamrat E, Tesfaye A, Gashaw Y, Biset S, Reta MA. Geteneh A, et al. Virol J. 2025 Apr 15;22(1):100. doi: 10.1186/s12985-025-02730-w. Virol J. 2025. PMID: 40234954 Free PMC article.
Multicentre Experience on the Diagnostic Yield of PCR Testing for Bacterial Pathogens in Sterile Site Infections.
Hasan MR, Tang P, Sharma A, Jayaratne P, Rutherford C, Sundararaju S, Suleiman M, Leto D, Yamamura D, Perez-Lopez A, Smieja M. Hasan MR, et al. J Assoc Med Microbiol Infect Dis Can. 2025 Jan 23;10(1):78-83. doi: 10.3138/jammi-2024-0030. eCollection 2025 Mar. J Assoc Med Microbiol Infect Dis Can. 2025. PMID: 40671851 Free PMC article.
Analytical and clinical validation of direct detection of antimicrobial resistance markers by plasma microbial cell-free DNA sequencing.
Christians FC, Akhund-Zade J, Jarman K, Venkatasubrahmanyam S, Noll N, Blauwkamp TA, Bercovici S, Zielinska A, Carr AL, Craney A, Pike M, Farrell JJ, Dadwal S, Wood JB, Matkovich E, McAdams S, Nolte FS. Christians FC, et al. J Clin Microbiol. 2024 Oct 16;62(10):e0042524. doi: 10.1128/jcm.00425-24. Epub 2024 Aug 28. J Clin Microbiol. 2024. PMID: 39194269 Free PMC article.

See all "Cited by" articles

References

1. Xu M., Qin X., Astion M.L., Rutledge J.C., Simpson J., Jerome K.R., Englund J.A., Zerr D.M., Migita R.T., Rich S., Childs J.C., Cent A., Del Beccaro M.A. Implementation of FilmArray respiratory viral panel in a core laboratory improves testing turnaround time and patient care. Am J Clin Pathol. 2013;139:118–123. - PMC - PubMed
1. Rogers B.B., Shankar P., Jerris R.C., Kotzbauer D., Anderson E.J., Watson J.R., O’Brien L.A., Uwindatwa F., McNamara K., Bost J.E. Impact of a rapid respiratory panel test on patient outcomes. Arch Pathol Lab Med. 2015;139:636–641. - PubMed
1. Subramony A., Zachariah P., Krones A., Whittier S., Saiman L. Impact of multiplex polymerase chain reaction testing for respiratory pathogens on healthcare resource utilization for pediatric inpatients. J Pediatr. 2016;173:196–201. - PMC - PubMed
1. Brendish N.J., Malachira A.K., Armstrong L., Houghton R., Aitken S., Nyimbili E., Ewings S., Lillie P.J., Clark T.W. Routine molecular point-of-care testing for respiratory viruses in adults presenting to hospital with acute respiratory illness (ResPOC): a pragmatic, open-label, randomised controlled trial. Lancet Respir Med. 2017;5:401–411. - PMC - PubMed
1. Hanson K.E., Azar M.M., Banerjee R., Chou A., Colgrove R.C., Ginocchio C.C., Hayden M.K., Holodiny M., Jain S., Koo S., Levy J., Timbrook T.T., Caliendo A.M. Molecular testing for acute respiratory tract infections: clinical and diagnostic recommendations from the IDSA’s diagnostics committee. Clin Infect Dis. 2020;71:2744–2751. - PMC - PubMed

Publication types

Actions
Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions

Grants and funding

P30 CA008748/CA/NCI NIH HHS/United States

LinkOut - more resources

Full Text Sources
Medical
- MedlinePlus Health Information

[1] Xu M., Qin X., Astion M.L., Rutledge J.C., Simpson J., Jerome K.R., Englund J.A., Zerr D.M., Migita R.T., Rich S., Childs J.C., Cent A., Del Beccaro M.A. Implementation of FilmArray respiratory viral panel in a core laboratory improves testing turnaround time and patient care. Am J Clin Pathol. 2013;139:118–123. - PMC - PubMed

[2] Xu M., Qin X., Astion M.L., Rutledge J.C., Simpson J., Jerome K.R., Englund J.A., Zerr D.M., Migita R.T., Rich S., Childs J.C., Cent A., Del Beccaro M.A. Implementation of FilmArray respiratory viral panel in a core laboratory improves testing turnaround time and patient care. Am J Clin Pathol. 2013;139:118–123. - PMC - PubMed

[3] Rogers B.B., Shankar P., Jerris R.C., Kotzbauer D., Anderson E.J., Watson J.R., O’Brien L.A., Uwindatwa F., McNamara K., Bost J.E. Impact of a rapid respiratory panel test on patient outcomes. Arch Pathol Lab Med. 2015;139:636–641. - PubMed

[4] Rogers B.B., Shankar P., Jerris R.C., Kotzbauer D., Anderson E.J., Watson J.R., O’Brien L.A., Uwindatwa F., McNamara K., Bost J.E. Impact of a rapid respiratory panel test on patient outcomes. Arch Pathol Lab Med. 2015;139:636–641. - PubMed

[5] Subramony A., Zachariah P., Krones A., Whittier S., Saiman L. Impact of multiplex polymerase chain reaction testing for respiratory pathogens on healthcare resource utilization for pediatric inpatients. J Pediatr. 2016;173:196–201. - PMC - PubMed

[6] Subramony A., Zachariah P., Krones A., Whittier S., Saiman L. Impact of multiplex polymerase chain reaction testing for respiratory pathogens on healthcare resource utilization for pediatric inpatients. J Pediatr. 2016;173:196–201. - PMC - PubMed

[7] Brendish N.J., Malachira A.K., Armstrong L., Houghton R., Aitken S., Nyimbili E., Ewings S., Lillie P.J., Clark T.W. Routine molecular point-of-care testing for respiratory viruses in adults presenting to hospital with acute respiratory illness (ResPOC): a pragmatic, open-label, randomised controlled trial. Lancet Respir Med. 2017;5:401–411. - PMC - PubMed

[8] Brendish N.J., Malachira A.K., Armstrong L., Houghton R., Aitken S., Nyimbili E., Ewings S., Lillie P.J., Clark T.W. Routine molecular point-of-care testing for respiratory viruses in adults presenting to hospital with acute respiratory illness (ResPOC): a pragmatic, open-label, randomised controlled trial. Lancet Respir Med. 2017;5:401–411. - PMC - PubMed

[9] Hanson K.E., Azar M.M., Banerjee R., Chou A., Colgrove R.C., Ginocchio C.C., Hayden M.K., Holodiny M., Jain S., Koo S., Levy J., Timbrook T.T., Caliendo A.M. Molecular testing for acute respiratory tract infections: clinical and diagnostic recommendations from the IDSA’s diagnostics committee. Clin Infect Dis. 2020;71:2744–2751. - PMC - PubMed

[10] Hanson K.E., Azar M.M., Banerjee R., Chou A., Colgrove R.C., Ginocchio C.C., Hayden M.K., Holodiny M., Jain S., Koo S., Levy J., Timbrook T.T., Caliendo A.M. Molecular testing for acute respiratory tract infections: clinical and diagnostic recommendations from the IDSA’s diagnostics committee. Clin Infect Dis. 2020;71:2744–2751. - PMC - PubMed

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Exploring the Utility of Multiplex Infectious Disease Panel Testing for Diagnosis of Infection in Different Body Sites: A Joint Report of the Association for Molecular Pathology, American Society for Microbiology, Infectious Diseases Society of America, and Pan American Society for Clinical Virology

Affiliations

Exploring the Utility of Multiplex Infectious Disease Panel Testing for Diagnosis of Infection in Different Body Sites: A Joint Report of the Association for Molecular Pathology, American Society for Microbiology, Infectious Diseases Society of America, and Pan American Society for Clinical Virology

Authors

Affiliations

Erratum in

Abstract

Conflict of interest statement

Similar articles

Cited by

References

Publication types

MeSH terms

Grants and funding

LinkOut - more resources

Full Text Sources

Medical

Erratum in

Abstract

Conflict of interest statement

Similar articles

Cited by

References

Publication types

MeSH terms

Related information

Grants and funding

LinkOut - more resources

Full Text Sources

Medical