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Review
. 2023 Dec;25(12):857-875.
doi: 10.1016/j.jmoldx.2023.08.005. Epub 2023 Sep 26.

Exploring the Utility of Multiplex Infectious Disease Panel Testing for Diagnosis of Infection in Different Body Sites: A Joint Report of the Association for Molecular Pathology, American Society for Microbiology, Infectious Diseases Society of America, and Pan American Society for Clinical Virology

Michael A Lewinski  1 , Kevin Alby  2 , N Esther Babady  3 , Susan M Butler-Wu  4 , Jennifer Dien Bard  5 , Alexander L Greninger  6 , Kimberly Hanson  7 , Samia N Naccache  8 , Duane Newton  9 , Robyn L Temple-Smolkin  10 , Frederick Nolte  11
Affiliations
Review

Exploring the Utility of Multiplex Infectious Disease Panel Testing for Diagnosis of Infection in Different Body Sites: A Joint Report of the Association for Molecular Pathology, American Society for Microbiology, Infectious Diseases Society of America, and Pan American Society for Clinical Virology

Michael A Lewinski et al. J Mol Diagn. 2023 Dec.

Erratum in

  • Corrections.
    [No authors listed] [No authors listed] J Mol Diagn. 2025 Mar;27(3):232. doi: 10.1016/j.jmoldx.2024.12.004. Epub 2025 Jan 16. J Mol Diagn. 2025. PMID: 39805327 Free PMC article. No abstract available.

Abstract

The use of clinical molecular diagnostic methods for detecting microbial pathogens continues to expand and, in some cases, supplant conventional identification methods in various scenarios. Analytical and clinical benefits of multiplex molecular panels for the detection of respiratory pathogens have been demonstrated in various studies. The use of these panels in managing different patient populations has been incorporated into clinical guidance documents. The Association for Molecular Pathology's Infectious Diseases Multiplex Working Group conducted a review of the current benefits and challenges to using multiplex PCR for the detection of pathogens from gastrointestinal tract, central nervous system, lower respiratory tract, and joint specimens. The Working Group also discusses future directions and novel approaches to detection of pathogens in alternate specimen types, and outlines challenges associated with implementation of these multiplex PCR panels.

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Conflict of interest statement

Disclosure Statement This article represents the consensus opinion of the Infectious Diseases Multiplex Working Group of the Clinical Practice Committee; all authors contributed equally. M.A.L. is employed by and owns stock options at Roche Molecular Systems. K.A. is employed by the University of North Carolina School of Medicine and has received honorarium from Cepheid. N.E.B. is employed by the Memorial Sloan Kettering Cancer Center and is a Clinical Advisory Board member at ArcBio and Scientific Advisory Board member at Bio-Rad Laboratories and Agena Bioscience. S.M.B.-W. is employed by the University of Southern California and has received consulting honoraria from Luminex and BioFire. J.D.B. is employed by the Children's Hospital of Los Angeles and the University of Southern California, is temporarily employed as a medical reviewer for GenMark Diagnostics, worked previously as a consultant and a member of the Expert Advisory panel for Accelerate Diagnostics, and is a current consultant for BioFire Diagnostics. A.L.G. is employed by the University of Washington Medical Center and is a recipient of research grants from Merck and Gilead and has central testing contracts with Abbott. S.N.N. is employed by and owns stock options at LabCorp Seattle. D.N. is employed by Bio-Rad Laboratories. F.N. is currently employed by Karius Inc. The remaining authors have declared no related conflicts of interests.

References

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