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. 2023 Sep 12;12(18):3400.
doi: 10.3390/foods12183400.

Combined Effect of High Hydrostatic Pressure and Proteolytic Fraction P1G10 from Vasconcellea cundinamarcensis Latex against Botrytis cinerea in Grape Juice

María José Torres-Ossandón  1   2   3 , Luis Castillo  2 , Elsa Uribe  3   4 , Cristina Bilbao-Sainz  5 , Kong Shun Ah-Hen  6 , Antonio Vega-Gálvez  3
Affiliations

Combined Effect of High Hydrostatic Pressure and Proteolytic Fraction P1G10 from Vasconcellea cundinamarcensis Latex against Botrytis cinerea in Grape Juice

María José Torres-Ossandón et al. Foods. .

Abstract

The effect of high hydrostatic pressure (HHP) and the proteolytic fraction P1G10 from papaya latex was studied to find out whether a synergy exists in the growth inhibition of Botrytis cinerea in grape juice, contributing to the improvement of conservation techniques and extending the shelf life and quality of food products. Grape juice (GJ) diluted to 16 °Brix with a water activity (aw) of 0.980 was prepared from a concentrated GJ and used in this study. Results indicated a 92% growth inhibition of B. cinerea when exposed to 1 mg/mL of P1G10 and 250 MPa/4 min of pressure treatment. The proximate composition and antioxidant compounds present in the GJ were not significantly affected after the treatments. Eight phenolic compounds and two flavonoids in GJ were identified and quantified, with values fluctuating between 12.77 ± 0.51 and 240.40 ± 20.9 mg/L in the control sample (0.1 MPa). The phenolic compounds showed a significant decrease after the applied treatments, with the HHP sample having a content of 65.4 ± 6.9 mg GAE/100 mL GJ. In conclusion, a synergistic effect at moderate HHP of 250 MPa/4 min with the addition of P1G10 was observed, and the successful development of a stable and acceptable GJ product was possible.

Keywords: bioactives in grape juice; food safety; growth inhibition of Botrytis; high-pressure processing; papaya antimicrobial peptide.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study, in the collection, analyses, or interpretation of data, in the writing of the manuscript, or in the decision to publish the results.

Figures

Figure 1
Figure 1
Plate count (a) and survival curves of B. cinerea (b) after HHP and combined HHP + P1G10 treatments. * Indicates significant differences (p < 0.05).
Figure 2
Figure 2
Antioxidant capacity (a) ORAC, (b) DPPH and contents of bioactives, (c) TFC, (d) TPC. Different letter indicates significant differences (p < 0.05).
Figure 3
Figure 3
Sensory evaluation: (a) Preference test for different attributes; (b) Hedonic scale for appreciation.
Figure 3
Figure 3
Sensory evaluation: (a) Preference test for different attributes; (b) Hedonic scale for appreciation.
See this image and copyright information in PMC

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