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. 2023 Sep 21;24(18):14363.
doi: 10.3390/ijms241814363.

Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis

Monika Wróbel  1 , Zielińska Zuzanna  2 , Łukasz Ołdak  2   3 , Aleksandra Kalicka  4 , Grzegorz Mańka  5 , Mariusz Kiecka  5 , Robert Z Spaczyński  6 , Piotr Piekarski  7 , Beata Banaszewska  8 , Artur Jakimiuk  9   10 , Tadeusz Issat  11 , Wojciech Rokita  12   13 , Jakub Młodawski  12   13 , Maria Szubert  14   15 , Piotr Sieroszewski  14   16 , Grzegorz Raba  17   18 , Kamil Szczupak  17   18 , Tomasz Kluz  19 , Marek Kluza  19 , Piotr Pierzyński  20 , Cezary Wojtyła  20 , Michał Lipa  21 , Damian Warzecha  20   21 , Mirosław Wielgoś  22   23 , Włodzimierz Sawicki  24 , Ewa Gorodkiewicz  3 , Piotr Laudański  20   24   25
Affiliations

Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis

Monika Wróbel et al. Int J Mol Sci. .

Abstract

Endometriosis is a chronic disease in which the endometrium cells are located outside the uterine cavity. The aim of this study was to evaluate circulating 20S proteasome and 20S immunoproteasome levels in plasma and peritoneal fluid in women with and without endometriosis in order to assess their usefulness as biomarkers of disease. Concentrations were measured using surface plasmon resonance imaging biosensors. Patients with suspected endometriosis were included in the study-plasma was collected in 112 cases and peritoneal fluid in 75. Based on the presence of endometriosis lesions detected during laparoscopy, patients were divided into a study group (confirmed endometriosis) and a control group (patients without endometriosis). Proteasome and immunoproteasome levels in both the plasma (p = 0.174; p = 0.696, respectively) and the peritoneal fluid (p = 0.909; p = 0.284, respectively) did not differ between those groups. There was a statistically significant difference in the plasma proteasome levels between patients in the control group and those with mild (Stage I and II) endometriosis (p = 0.047) and in the plasma immunoproteasome levels in patients with ovarian cysts compared to those without (p = 0.017). The results of our study do not support the relevance of proteasome and immunoproteasome determination as biomarkers of the disease but suggest a potentially active role in the pathogenesis of endometriosis.

Keywords: endometriosis; immunoproteasome; peritoneal fluid; plasma; proteasome.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Distribution of concentrations of the studied parameters in plasma.
Figure 2
Figure 2
Distribution of concentrations of the studied parameters in peritoneal fluid.
Figure 3
Figure 3
Differences in plasma concentrations of the studied parameters according to the stage of endometriosis.
Figure 4
Figure 4
Differences in peritoneal fluid concentrations of the studied parameters according to the stage of endometriosis.
Figure 5
Figure 5
Calibration curve for immunoproteasome 20Si. Concentration of inhibitor = 15 µg/mL, pH = 7.4.
Figure 6
Figure 6
Calibration curve of the sum of the 20S proteasome and the 20Si immunoproteasome. Concentration of inhibitor = 15 µg/mL, pH = 7.4.
See this image and copyright information in PMC

References

    1. Bulun S.E., Yilmaz B.D., Sison C., Miyazaki K., Bernardi L., Liu S., Kohlmeier A., Yin P., Milad M., Wei J. Endometriosis. Endocr. Rev. 2019;40:1048–1079. doi: 10.1210/er.2018-00242. - DOI - PMC - PubMed
    1. Zondervan K.T., Becker C.M., Missmer S.A. Endometriosis. N. Engl. J. Med. 2020;382:1244–1256. doi: 10.1056/NEJMra1810764. - DOI - PubMed
    1. Wrobel M., Wielgos M., Laudanski P. Corrigendum to “Diagnostic delay of endometriosis in adults and adolescence-current stage of knowledge” [Adv. Med. Sci. (2022 Mar) 67 (1) 148–153] Adv. Med. Sci. 2023;68:60. doi: 10.1016/j.advms.2022.12.004. - DOI - PubMed
    1. Riazi H., Tehranian N., Ziaei S., Mohammadi E., Hajizadeh E., Montazeri A. Clinical diagnosis of pelvic endometriosis: A scoping review. BMC Womens Health. 2015;15:39. doi: 10.1186/s12905-015-0196-z. - DOI - PMC - PubMed
    1. Sampson J.A. Peritoneal endometriosis due to the menstrual dissemination of endometrial tissue into the peritoneal cavity. Am. J. Obstet. Gynecol. 1927;14:422–469. doi: 10.1016/S0002-9378(15)30003-X. - DOI

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