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. 2023 Sep 20;59(9):1687.
doi: 10.3390/medicina59091687.

The Effect of Moderate-Intensity Physical Exercise on Some Serum Inflammation Markers and the Immune System in Rats Fed Intermittent Fasting with a High-Fat Diet

Nizamettin Günbatar  1 , Bahattin Bulduk  1 , Selver Bezgin  1 , Gökhan Oto  2 , Fahri Bayıroğlu  3 , Mehmet Bulduk  1
Affiliations

The Effect of Moderate-Intensity Physical Exercise on Some Serum Inflammation Markers and the Immune System in Rats Fed Intermittent Fasting with a High-Fat Diet

Nizamettin Günbatar et al. Medicina (Kaunas). .

Abstract

Background and Objectives: This study aimed to investigate the impact of moderate-intensity physical exercise on serum inflammation markers and the immune system in rats that were fed a high-fat diet (HFD) with intermittent fasting. Materials and Methods: A total of 48 Wistar albino male rats were included in the study and divided into eight groups, each consisting of six rats. Group 1 served as the control group (CG), receiving a standard diet. Group 2 followed the standard nutrition program with intermittent fasting (CG + IF). Group 3 underwent exercise with a standard diet (CG + E). Group 4 underwent both a standard diet with intermittent fasting and exercise (CG + IF + E). Group 5 was fed a high-fat diet (HFD). Group 6 received a high-fat diet with intermittent fasting (HFD + IF). Group 7 followed a high-fat diet with exercise (HFD + E). Group 8 underwent both a high-fat diet with intermittent fasting and exercise (HFD + IF + E). The study lasted for 8 weeks. Results: The results of the analysis show that lymphocyte cell levels in groups HFD + IF, HFD + IF, and HFD + IF + E were higher compared to groups CG-HFD (p < 0.05). Additionally, B lymphocyte and monocyte cell levels were higher in group HFD + IF + E compared to groups CG, CG + IF, and CG + IF + E, as well as CG, CG + IF, and CG + E, respectively. TNF-α levels were significantly higher in group HFD compared to the other groups. Furthermore, IL 10 levels were higher in group HFD + IF + E compared to the other groups. Conclusions: These findings indicate that moderate exercise and intermittent fasting, particularly in groups fed a high-fat diet, increased anti-inflammatory cytokine levels, and certain immune system cell counts, while decreasing pro-inflammatory cytokine levels.

Keywords: exercise; highfat diet; intermittent fasting; monocytes.

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Conflict of interest statement

The authors declare that there is no conflict of interest.

Figures

Figure 1
Figure 1
The mean values of, naturel killer (A), ymphocyte (B), B-lymphocyte (C), and WBC (D) among the groups. # p: significant (p < 0.05).
Figure 2
Figure 2
The mean values of T–helper (A), monocyte (B), IL-10 (C), TNF-α,and (D) among the groups. # p: significant (p < 0.05).
Figure 3
Figure 3
The mean values of IGF-1 (A), IL 4 (B), IL-12 (C), neutrophils, and (D) among the groups.
Figure 4
Figure 4
The mean values of eosinophils (A), basophils (B), IL-1β, and (C) among the groups.
Figure 5
Figure 5
Flow cytometry gating procedure.
See this image and copyright information in PMC

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