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. 2024 Jan;19(1):e2300246.
doi: 10.1002/biot.202300246. Epub 2023 Oct 6.

The expression pattern of cytokeratin 6a in epithelial cells of different origin in dermo-epidermal skin substitutes in vivo

Katarzyna Michalak-Micka  1   2 , Celina Tenini  1   2 , Sophie Böttcher-Haberzeth  1   2   3 , Luca Mazzone  2   4   5 , Luca Pontiggia  1   2 , Agnes S Klar  1   2 , Ueli Moehrlen  2   3   4   5 , Thomas Biedermann  1   2   3
Affiliations

The expression pattern of cytokeratin 6a in epithelial cells of different origin in dermo-epidermal skin substitutes in vivo

Katarzyna Michalak-Micka et al. Biotechnol J. 2024 Jan.

Abstract

Keratinocytes are the predominant cell type of skin epidermis. Through the programmed process of differentiation, they form a cornified envelope that provides a physical protective barrier against harmful external environment. Keratins are major structural proteins of keratinocytes that together with actin filaments and microtubules form the cytoskeleton of these cells. In this study, we examined the expression pattern and distribution of cytokeratin 6a (CK6a) in healthy human skin samples of different body locations, in fetal and scar skin samples, as well as in dermo-epidermal skin substitutes (DESSs). We observed that CK6a expression is significantly upregulated in fetal skin and scar tissue as well as in skin grafts after short-term transplantation. Importantly, the abundance of CK6a corresponds directly to the expression pattern of wound healing marker CK16. We postulate that CK6a is a useful marker to accurately evaluate the homeostatic state of DESSs.

Keywords: cytokeratin 6a; dermo-epidermal skin substitutes; epidermis; homeostasis; wound healing.

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