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. 2023 Jun 27:8:278.
doi: 10.12688/wellcomeopenres.19624.1. eCollection 2023.

The genome sequence of the Dark Spectacle, Abrostola triplasia (Linnaeus, 1758)

Douglas Boyes  1 , Owen T Lewis  2 ; University of Oxford and Wytham Woods Genome Acquisition Lab; Darwin Tree of Life Barcoding collective; Wellcome Sanger Institute Tree of Life programme; Wellcome Sanger Institute Scientific Operations: DNA Pipelines collective; Tree of Life Core Informatics collective; Darwin Tree of Life Consortium
Affiliations

The genome sequence of the Dark Spectacle, Abrostola triplasia (Linnaeus, 1758)

Douglas Boyes et al. Wellcome Open Res. .

Abstract

We present a genome assembly from an individual male Abrostola triplasia (the Dark Spectacle; Arthropoda; Insecta; Lepidoptera; Noctuidae). The genome sequence is 362.7 megabases in span. Most of the assembly is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled and is 15.34 kilobases in length. Gene annotation of this assembly on Ensembl identified 11,532 protein coding genes.

Keywords: Abrostola triplasia; Dark Spectacle; Lepidoptera; chromosomal; genome sequence.

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Conflict of interest statement

No competing interests were disclosed.

Figures

Figure 1.
Figure 1.. Photograph of the Abrostola triplasia (ilAbrTril1) specimen used for genome sequencing.
Figure 2.
Figure 2.. Genome assembly of Abrostola triplasia, ilAbrTril1.1: metrics.
The BlobToolKit Snailplot shows N50 metrics and BUSCO gene completeness. The main plot is divided into 1,000 size-ordered bins around the circumference with each bin representing 0.1% of the 362,722,748 bp assembly. The distribution of scaffold lengths is shown in dark grey with the plot radius scaled to the longest scaffold present in the assembly (21,208,463 bp, shown in red). Orange and pale-orange arcs show the N50 and N90 scaffold lengths (12,758,438 and 8,625,622 bp), respectively. The pale grey spiral shows the cumulative scaffold count on a log scale with white scale lines showing successive orders of magnitude. The blue and pale-blue area around the outside of the plot shows the distribution of GC, AT and N percentages in the same bins as the inner plot. A summary of complete, fragmented, duplicated and missing BUSCO genes in the lepidoptera_odb10 set is shown in the top right. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/ilAbrTril1.1/dataset/CAMIUI01/snail.
Figure 3.
Figure 3.. Genome assembly of Abrostola triplasia, ilAbrTril1.1: BlobToolKit GC-coverage plot.
Scaffolds are coloured by phylum. Circles are sized in proportion to scaffold length. Histograms show the distribution of scaffold length sum along each axis. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/ilAbrTril1.1/dataset/CAMIUI01/blob.
Figure 4.
Figure 4.. Genome assembly of Abrostola triplasia, ilAbrTril1.1: BlobToolKit cumulative sequence plot.
The grey line shows cumulative length for all scaffolds. Coloured lines show cumulative lengths of scaffolds assigned to each phylum using the buscogenes taxrule. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/ilAbrTril1.1/dataset/CAMIUI01/cumulative.
Figure 5.
Figure 5.. Genome assembly of Abrostola triplasia, ilAbrTril1.1: Hi-C contact map of the ilAbrTril1.1 assembly, visualised using HiGlass.
Chromosomes are shown in order of size from left to right and top to bottom. An interactive version of this figure may be viewed at https://genome-note-higlass.tol.sanger.ac.uk/l/?d=XXUZC-xDR-uMmH0_Av5lzg.
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