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. 2023 Jun 22:8:271.
doi: 10.12688/wellcomeopenres.19585.1. eCollection 2023.

The genome sequence of the spider, Parasteatoda lunata (Clerck, 1757)

Geoff Oxford  1 ; Natural History Museum Genome Acquisition Lab; Darwin Tree of Life Barcoding collective; Wellcome Sanger Institute Tree of Life programme; Wellcome Sanger Institute Scientific Operations: DNA Pipelines collective; Tree of Life Core Informatics collective; Darwin Tree of Life Consortium
Affiliations

The genome sequence of the spider, Parasteatoda lunata (Clerck, 1757)

Geoff Oxford et al. Wellcome Open Res. .

Abstract

We present a genome assembly from an individual female Parasteatoda lunata (spider; Arthropoda; Arachnida; Araneae; Theridiidae). The genome sequence is 1,411.4 megabases in span. Most of the assembly is scaffolded into 12 chromosomal, including the X 1 and X 2 sex chromosomes. The mitochondrial genome has also been assembled and is 14.29 kilobases in length.

Keywords: Araneae; Parasteatoda lunata; chromosomal; genome sequence; spider.

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Conflict of interest statement

No competing interests were disclosed.

Figures

Figure 1.
Figure 1.
Photographs of Parasteatoda lunata individuals from the New Earswick population A. Female spider in retreat, B. Retreat and egg sac, C. Female spider in web, D. P. lunata (qqParLuna2) specimen during preservation and processing. (Photographs A– C by Geoff Oxford.)
Figure 2.
Figure 2.. Genome assembly of Parasteatoda lunata, qqParLuna2.1: metrics.
The BlobToolKit Snailplot shows N50 metrics and BUSCO gene completeness. The main plot is divided into 1,000 size-ordered bins around the circumference with each bin representing 0.1% of the 1,411,378,570 bp assembly. The distribution of scaffold lengths is shown in dark grey with the plot radius scaled to the longest scaffold present in the assembly (134,647,540 bp, shown in red). Orange and pale-orange arcs show the N50 and N90 scaffold lengths (114,314,067 and 104,696,160 bp), respectively. The pale grey spiral shows the cumulative scaffold count on a log scale with white scale lines showing successive orders of magnitude. The blue and pale-blue area around the outside of the plot shows the distribution of GC, AT and N percentages in the same bins as the inner plot. A summary of complete, fragmented, duplicated and missing BUSCO genes in the arachnida_odb10 set is shown in the top right. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/qqParLuna2.1/dataset/CASBRV01/snail.
Figure 3.
Figure 3.. Genome assembly of Parasteatoda lunata, qqParLuna2.1: BlobToolKit GC-coverage plot.
Scaffolds are coloured by phylum. Circles are sized in proportion to scaffold length. Histograms show the distribution of scaffold length sum along each axis. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/qqParLuna2.1/dataset/CASBRV01/blob.
Figure 4.
Figure 4.. Genome assembly of Parasteatoda lunata, qqParLuna2.1: BlobToolKit cumulative sequence plot.
The grey line shows cumulative length for all scaffolds. Coloured lines show cumulative lengths of scaffolds assigned to each phylum using the buscogenes taxrule. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/qqParLuna2.1/dataset/CASBRV01/cumulative.
Figure 5.
Figure 5.. Genome assembly of Parasteatoda lunata, qqParLuna2.1: Hi-C contact map of the qqParLuna2.1 assembly, visualised using HiGlass.
Chromosomes are shown in order of size from left to right and top to bottom. An interactive version of this figure may be viewed at https://genome-note-higlass.tol.sanger.ac.uk/l/?d=UJaCEon1R-OQIOKvaJNE0g.
See this image and copyright information in PMC

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