Draft genomes of Aeromonas caviae from patients with cholera-like illness during the 2022-2023 cholera outbreak in Malawi

Khuzwayo C Jere^{1

2

3

4}, Innocent Chibwe⁵, David Chaima⁶, Watipaso Kasambala⁵, Chimwemwe Mhango^{2

7}, Joseph Bitilinyu-Bangoh⁵, Bernard Mvula⁵, Wakisa Kipandula³, Arox W Kamng'ona⁷, A Duncan Steele⁸, Annie Chauma-Mwale⁵, Daniel Hungerford^{1

4}, Matthew Kagoli⁵, Martin M Nyaga⁹, Queen Dube¹⁰, Neil French^{1

6}, Nigel A Cunliffe^{1

4}, Chisomo L Msefula^{4

6}, Chrispin Chaguza^{11

12

13

14}

Affiliations

¹ Department of Clinical Infection, Microbiology and Immunology, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, United Kingdom.
² Malawi-Liverpool-Wellcome Research Programme, Kamuzu University of Health Sciences, Blantyre, Malawi.
³ Department of Medical Laboratory Sciences, Faculty of Biomedical Sciences and Health Profession, Kamuzu University of Health Sciences, Blantyre, Malawi.
⁴ NIHR Global Health Research Group on Gastrointestinal Infections, University of Liverpool, Liverpool, Merseyside, United Kingdom.
⁵ National Microbiology Reference Laboratory, Public Health Institute of Malawi, Lilongwe, Malawi.
⁶ Department of Pathology, School of Medicine and Oral Health, Kamuzu University of Health Sciences, Blantyre, Malawi.
⁷ Department of Biomedical Sciences, School of Life Sciences and Allied Health Professions, Kamuzu University of Health Sciences, Blantyre, Malawi.
⁸ Diarrhoeal Pathogens Research Unit, Sefako Makgatho Health Sciences University, Medunsa, Pretoria, South Africa.
⁹ Next Generation Sequencing Unit and Division of Virology, Faculty of Health Sciences, University of the Free State, Bloemfontein, Free State, South Africa.
¹⁰ Malawi Ministry of Health, Lilongwe, Malawi.
¹¹ Department of Epidemiology of Microbial Diseases, Yale School of Public Health, YaleUniversity, New Haven, Connecticut, USA.
¹² Yale Institute for Global Health, Yale University, New Haven, Connecticut, USA.
¹³ NIHR Mucosal Pathogens Research Unit, Research Department of Infection, Division of Infection and Immunity, University College London, London, United Kingdom.
¹⁴ Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, United Kingdom.

PMID: 37768056
PMCID: PMC10586119
DOI: 10.1128/MRA.00580-23

Draft genomes of Aeromonas caviae from patients with cholera-like illness during the 2022-2023 cholera outbreak in Malawi

Khuzwayo C Jere et al. Microbiol Resour Announc. 2023.

. 2023 Oct 19;12(10):e0058023.

doi: 10.1128/MRA.00580-23. Epub 2023 Sep 28.

Authors

Affiliations

¹ Department of Clinical Infection, Microbiology and Immunology, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, United Kingdom.
² Malawi-Liverpool-Wellcome Research Programme, Kamuzu University of Health Sciences, Blantyre, Malawi.
³ Department of Medical Laboratory Sciences, Faculty of Biomedical Sciences and Health Profession, Kamuzu University of Health Sciences, Blantyre, Malawi.
⁴ NIHR Global Health Research Group on Gastrointestinal Infections, University of Liverpool, Liverpool, Merseyside, United Kingdom.
⁵ National Microbiology Reference Laboratory, Public Health Institute of Malawi, Lilongwe, Malawi.
⁶ Department of Pathology, School of Medicine and Oral Health, Kamuzu University of Health Sciences, Blantyre, Malawi.
⁷ Department of Biomedical Sciences, School of Life Sciences and Allied Health Professions, Kamuzu University of Health Sciences, Blantyre, Malawi.
⁸ Diarrhoeal Pathogens Research Unit, Sefako Makgatho Health Sciences University, Medunsa, Pretoria, South Africa.
⁹ Next Generation Sequencing Unit and Division of Virology, Faculty of Health Sciences, University of the Free State, Bloemfontein, Free State, South Africa.
¹⁰ Malawi Ministry of Health, Lilongwe, Malawi.
¹¹ Department of Epidemiology of Microbial Diseases, Yale School of Public Health, YaleUniversity, New Haven, Connecticut, USA.
¹² Yale Institute for Global Health, Yale University, New Haven, Connecticut, USA.
¹³ NIHR Mucosal Pathogens Research Unit, Research Department of Infection, Division of Infection and Immunity, University College London, London, United Kingdom.
¹⁴ Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, United Kingdom.

PMID: 37768056
PMCID: PMC10586119
DOI: 10.1128/MRA.00580-23

Abstract

Aeromonas caviae is an increasingly recognized etiological agent of acute gastroenteritis. Here, we report five draft genomes of A. caviae isolated from suspected cholera cases during the 2022-2023 cholera outbreak in Malawi.

Keywords: Aeromonas caviae; Malawi; cholera outbreak; cholera-like illness; sub-Saharan Africa; whole-genome sequence.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

**Fig 1**
Maximum likelihood phylogeny of *A. caviae* isolated from cholera-like illness in Malawi in the context of the publicly available genomes in the GenBank database. The phylogeny was generated based on 383,314 SNPs using FastTree. The tree was rooted on the branch separating the *A. caviae* genomes from the outgroup sequence for *A. sanarellii* strain MaGu-431 (GenBank assembly accession: GCF_017815105). The taxon labels for the *A. caviae* genomes from Malawi are shown in bold text in the phylogeny. For clarity, the outgroup is not shown in the phylogenetic tree.

See this image and copyright information in PMC

References

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Draft genomes of Aeromonas caviae from patients with cholera-like illness during the 2022-2023 cholera outbreak in Malawi

Affiliations

Draft genomes of Aeromonas caviae from patients with cholera-like illness during the 2022-2023 cholera outbreak in Malawi

Authors

Affiliations

Abstract

Conflict of interest statement

Figures

References

Grants and funding

LinkOut - more resources

Full Text Sources