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Review
. 2024 Jan-Feb;16(1):e1630.
doi: 10.1002/wsbm.1630. Epub 2023 Sep 28.

iPSCs as a groundbreaking tool for the study of adverse drug reactions: A new avenue for personalized therapy

Paola Rispoli  1 , Tatiana Scandiuzzi Piovesan  1 , Giuliana Decorti  1   2 , Gabriele Stocco  1   2 , Marianna Lucafò  3
Affiliations
Review

iPSCs as a groundbreaking tool for the study of adverse drug reactions: A new avenue for personalized therapy

Paola Rispoli et al. WIREs Mech Dis. 2024 Jan-Feb.

Abstract

Induced pluripotent stem cells (iPSCs), obtained by reprogramming different somatic cell types, represent a promising tool for the study of drug toxicities, especially in the context of personalized medicine. Indeed, these cells retain the same genetic heritage of the donor, allowing the development of personalized models. In addition, they represent a useful tool for the study of adverse drug reactions (ADRs) in special populations, such as pediatric patients, which are often poorly represented in clinical trials due to ethical issues. Particularly, iPSCs can be differentiated into any tissue of the human body, following several protocols which use different stimuli to induce specific differentiation processes. Differentiated cells also maintain the genetic heritage of the donor, and therefore are suitable for personalized pharmacological studies; moreover, iPSC-derived differentiated cells are a valuable tool for the investigation of the mechanisms underlying the physiological differentiation processes. iPSCs-derived organoids represent another important tool for the study of ADRs. Precisely, organoids are in vitro 3D models which better represent the native organ, both from a structural and a functional point of view. Moreover, in the same way as iPSC-derived 2D models, iPSC-derived organoids are appropriate personalized models since they retain the genetic heritage of the donor. In comparison to other in vitro models, iPSC-derived organoids present advantages in terms of versatility, patient-specificity, and ethical issues. This review aims to provide an updated report of the employment of iPSCs, and 2D and 3D models derived from these, for the study of ADRs. This article is categorized under: Cancer > Stem Cells and Development.

Keywords: adverse drug reactions; induced pluripotent stem cells; medicine; organoids; personalized; pharmacology.

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References

FURTHER READING
    1. Bernal, J. A. (2013). RNA-based tools for nuclear reprogramming and lineage-conversion: Towards clinical applications. Journal of Cardiovascular Translational Research, 6(6), 956-968. https://doi.org/10.1007/s12265-013-9494-8
    1. Chen, G., Guo, Y., Li, C., Li, S., & Wan, X. (2020). Small molecules that promote self-renewal of stem cells and somatic cell reprogramming. Stem Cell Reviews and Reports, 16(3), 511-523. https://doi.org/10.1007/s12015-020-09965-w
    1. Hu, K. (2014). Vectorology and factor delivery in induced pluripotent stem cell reprogramming. Stem Cells and Development, 23(12), 1301-1315. https://doi.org/10.1089/scd.2013.0621
    1. Miyoshi, N., Ishii, H., Nagano, H., Haraguchi, N., Dewi, D. L., Kano, Y., Nishikawa, S., Tanemura, M., Mimori, K., Tanaka, F., Saito, T., Nishimura, J., Takemasa, I., Mizushima, T., Ikeda, M., Yamamoto, H., Sekimoto, M., Doki, Y., & Mori, M. (2011). Reprogramming of mouse and human cells to pluripotency using mature microRNAs. Cell Stem Cell, 8(6), 633-638. https://doi.org/10.1016/j.stem.2011.05.001
    1. Nordin, F., Ahmad, R. N. R., & Farzaneh, F. (2017). Transactivator protein: An alternative for delivery of recombinant proteins for safer reprogramming of induced pluripotent stem cell. Virus Research, 235, 106-114. https://doi.org/10.1016/j.virusres.2017.04.007
REFERENCES
    1. Alempijevic, T., Zec, S., & Milosavljevic, T. (2017). Drug-induced liver injury: Do we know everything? World Journal of Hepatology, 9(10), 491-502. https://doi.org/10.4254/wjh.v9.i10.491
    1. Aminkeng, F., Ross, C. J. D., Rassekh, S. R., Hwang, S., Rieder, M. J., Bhavsar, A. P., Smith, A., Sanatani, S., Gelmon, K. A., Bernstein, D., Hayden, M. R., Amstutz, U., Carleton, B. C., & CPNDS Clinical Practice Recommendations Group. (2016). Recommendations for genetic testing to reduce the incidence of anthracycline-induced cardiotoxicity. British Journal of Clinical Pharmacology, 82(3), 683-695. https://doi.org/10.1111/bcp.13008
    1. Andersen, J., Revah, O., Miura, Y., Thom, N., Amin, N. D., Kelley, K. W., Singh, M., Chen, X., Thete, M. V., Walczak, E. M., Vogel, H., Fan, H. C., & Paşca, S. P. (2020). Generation of functional human 3D cortico-motor assembloids. Cell, 183(7), 1913-1929.e26. https://doi.org/10.1016/j.cell.2020.11.017
    1. Annand, R. R. (2021). Generation of human iPSCs by reprogramming with the unmodified synthetic mRNA. Methods in Molecular Biology, 2239, 163-174. https://doi.org/10.1007/978-1-0716-1084-8_11
    1. Azar, J., Bahmad, H. F., Daher, D., Moubarak, M. M., Hadadeh, O., Monzer, A., Al Bitar, S., Jamal, M., Al-Sayegh, M., & Abou-Kheir, W. (2021). The use of stem cell-derived organoids in disease modeling: An update. International Journal of Molecular Sciences, 22(14), 7667. https://doi.org/10.3390/ijms22147667

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