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. 2023 Sep 29;205(10):346.
doi: 10.1007/s00203-023-03682-x.

Flavobacterium okayamense sp. nov. isolated from surface seawater

Kei Kitahara  1 , Basilua Andre Muzembo  2 , Sho Morohoshi  3 , Tadao Kunihiro  3 , Nozomi Tazato  3 , Ayumu Ohno  2 , Kazuma Uesaka  4 , Makoto Taniguchi  5 , Shin-Ichi Miyoshi  2
Affiliations

Flavobacterium okayamense sp. nov. isolated from surface seawater

Kei Kitahara et al. Arch Microbiol. .

Abstract

Strain KK2020170T, a Gram-stain negative, yellow colony-forming bacterium, was isolated from surface seawater sampled in Kojima Bay, Okayama, Japan. Phylogenetic analysis based on the 16S rRNA gene revealed that strain KK2020170T belongs to the genus Flavobacterium, with Flavobacterium haoranii LQY-7T (98.1% similarity) being its closest relative, followed by Flavobacterium sediminis MEBiC07310T (96.9%) and Flavobacterium urocaniciphilum YIT 12746T (96.0%). Whole-genome shotgun sequencing showed that strain KK2020170T, when paralleled with F. haoranii LQY-7 T, had 81.3% average nucleotide identity, and 24.6% in silico DNA-DNA hybridization values, respectively. The DNA G + C content of strain KK2020170T was 31.1 mol%. The most abundant fatty acids (> 10%) of strain KK2020170T were iso-C15: 0, iso-C17: 0 3-OH and iso-C15: 1 G. The dominant respiratory quinone of the strain was menaquinone MK-6. Based on the phylogenetic and phenotypic analysis results, we propose that strain KK2020170T represents a novel species, for which the name Flavobacterium okayamense sp. nov. has been proposed. The type strain is KK2020170T (= ATCC TSD-280 T = NBRC 115344 T).

Keywords: Bacteroidota; Flavobacterium; New taxa; Sea water.

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Conflict of interest statement

The authors declare that there were no conflicts of interest.

Figures

Fig. 1
Fig. 1
Neighbor-joining tree depicting the evolutionary relationships of F. okayamense strain KK2020170T and 37 adjacent type strains in the genus Flavobacterium based on 16S rRNA gene sequences. Bootstrap values (given as percentages of 1000 replicates) with > 50% values are displayed. Flavobacterium aquatile is the type species in the genus Flavobacterium. Myroides odoratus NBRC 14945 T (AB517709) was employed as an outgroup. The bar represents a Knuc distance of 0.01
See this image and copyright information in PMC

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