Aromatic compounds from the seeds of Dolichos lablab L. with anti-inflammatory activity

Abstract

Twenty-four aromatic compounds including five novel ones, dolilabphenosides A (1), B₁ (2), B₂ (3), C₁ (4), and C₂ (5) were obtained from the seeds of Dolichos lablab L. Their structures were established based on spectroscopic analyses and chemical reactions. Among the known compounds, 9, 10, 14, 17, 19, and 22-24 were gained from the family Leguminosae for the first time, and 6, 8, 11-13, 15, 16, 18, 20, as well as 21 were firstly identified from Dolichos genus. Moreover, the inhibitory effect evaluation of all the isolates against LPS-induced nitric oxide (NO) production in RAW264.7 macrophages suggested that compounds 1-3, 6, 7, 11-15, 17, 20, 21, 23, 24 exhibited anti-inflammatory activity in a concentration-dependent manner. Moreover, the novel compounds, dolilabphenosides A (1), B₁ (2), B₂ (3) were found to inhibit the secretion of inflammatory cytokine IL-1β.

Keywords: Aromatic compounds; IL-1β; NO production inhibition; The seeds of Dolichos lablab L..