Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Jul 13;15(4):545-551.
doi: 10.1055/s-0043-1768951. eCollection 2023 Dec.

Martin's Formula As the Most Suitable Method for Estimation of Low-Density Lipoprotein Cholesterol in Indian Population

Fatima Farheen  1 , Sudha Ambiger  2 , Kamarudin Jaalam  3 , Shivalingappa Javali  4
Affiliations

Martin's Formula As the Most Suitable Method for Estimation of Low-Density Lipoprotein Cholesterol in Indian Population

Fatima Farheen et al. J Lab Physicians. .

Abstract

Background Because of cost effectiveness, most of the laboratories in India estimate low-density lipoprotein cholesterol (LDL-C) levels with the Friedewald's formula. There were many shortcomings of the Friedewald's formula. Recently, Martin and colleagues have derived a new formula for calculating LDL-C. The present study was undertaken to calculate LDL-C using various formulae (Friedewald's formula, Anandaraja's formula, and Martin's formula) and to compare directly measured LDL-C (D-LDL-C) with calculated LDL-C at various ranges of triglyceride (TG) concentration. Materials and Methods The present study compared LDL-C measured by Martin's formula, Friedewald's formula, and Anandaraja's formula with D-LDL-C in 280 outpatient fasting samples between the age groups of 18 and 50 years. Depending on the TG values, study samples were divided into four groups. Group 1: less than 200 mg/dL; Group 2: 200 to 300 mg/dL; Group 3: 300 to 400 mg/dL; and Group 4: more than 400 mg/dL. Results Martin's formula shows highest correlation with r -value of 0.9979 compared with Friedewald's (0.9857) and Anandaraja's (0.9683) r -values. The mean difference was least for Martin's formula (0.31 ± 3.53) compared with other formulae. Among all the groups, percentage of error was least for Martin's formula (0.23%). Martin's LDL-C shows highest concordance (90.90%) compared with Friedewald's (79.60%) and Anandaraja's formulae (82.90%). Conclusion Among all the groups, Martin's formula shows highest correlation, least percentage of error, highest concordance, and least mean differences. At all TG levels, Martin's formula is the best formula compared with the Friedewald's formula and Anandaraja's formula.

Keywords: Anandaraja's formula; Friedewald's formula; LDL-C; Martin's formula.

PubMed Disclaimer

Conflict of interest statement

Conflict of Interest None declared

Figures

Fig. 1
Fig. 1
( A–C ) Correlation between direct low-density lipoprotein-cholesterol (LDL-C) and calculated LDL-C.
See this image and copyright information in PMC

References

    1. Talwalkar P G, Sreenivas C G, Gulati A, Baxi H. Journey in guidelines for lipid management: from adult treatment panel (ATP)-I to ATP-III and what to expect in ATP-IV. Indian J Endocrinol Metab. 2013;17(04):628–635. - PMC - PubMed
    1. Badrakiya K M, Shah A D, Makadia M G. Comparison of LDL-cholesterol estimated by direct method and by calculation. IJBAR. 2016;7(08):353–358.
    1. Schaefer E J, Otokozawa S, Ai M.Limitations of direct methods and the reference method for measuring HDL and LDL cholesterol Clin Chem 201157071081–1083., author reply 1083 - PubMed
    1. Friedewald W T, Levy R I, Fredrickson D S. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972;18(06):499–502. - PubMed
    1. Anandaraja S, Narang R, Godeswar R, Laksmy R, Talwar K K. Low-density lipoprotein cholesterol estimation by a new formula in Indian population. Int J Cardiol. 2005;102(01):117–120. - PubMed