Regulation of production of embryonic chick liver delta-aminolevulinate synthase: effects of testosterone and of hemin on the mRNA of the enzyme
- PMID: 3778461
- DOI: 10.1016/0006-291x(86)91060-0
Regulation of production of embryonic chick liver delta-aminolevulinate synthase: effects of testosterone and of hemin on the mRNA of the enzyme
Abstract
The effects of testosterone and of hemin on the concentration of the mRNA of embryonic chick liver ALA synthase were investigated. Using cDNA-RNA liquid hybridization analyses, we determined that testosterone, when injected into the fluid surrounding chick embryos, caused a dose-dependent increase in the concentration of ALA synthase mRNA in liver. Similarly, addition of testosterone (5 micrograms/ml) or of 75 micrograms/ml of allylisopropylacetamide (AIA) into the medium of chick embryo hepatocytes maintained in culture caused an increase in the concentration of ALA synthase mRNA. Hemin (2 or 5 microM), when added to the culture medium, inhibited the elevations of ALA synthase mRNA concentration brought on by testosterone and by AIA.
Similar articles
-
Regulation of production of delta-aminolaevulinate synthase in tissues of chick embryos. Effects of porphyrogenic agents and of haem precursors.Biochem J. 1989 Sep 15;262(3):815-21. doi: 10.1042/bj2620815. Biochem J. 1989. PMID: 2590168 Free PMC article.
-
Biogenesis of embryonic chick liver delta-aminolevulinate synthase: regulation of the level of mRNA by hemin.Arch Biochem Biophys. 1987 Mar;253(2):297-304. doi: 10.1016/0003-9861(87)90182-2. Arch Biochem Biophys. 1987. PMID: 3566276
-
Regulation of the stability of chicken embryo liver delta-aminolevulinate synthase mRNA by hemin.Biochem Biophys Res Commun. 1989 Jul 14;162(1):102-7. doi: 10.1016/0006-291x(89)91968-2. Biochem Biophys Res Commun. 1989. PMID: 2751643
-
Regulation by heme of synthesis and intracellular translocation of delta-aminolevulinate synthase in the liver.Mol Cell Biochem. 1981 Jun 9;37(1):27-41. doi: 10.1007/BF02355885. Mol Cell Biochem. 1981. PMID: 6789140 Review. No abstract available.
-
[Control of the amount of delta-aminolevulinate synthase in liver mitochondria (author's transl)].Seikagaku. 1979 Jun;51(6):420-36. Seikagaku. 1979. PMID: 226633 Review. Japanese. No abstract available.
Cited by
-
Expression of 5-aminolaevulinate synthase and cytochrome P-450 mRNAs in chicken embryo hepatocytes in vivo and in culture. Effect of porphyrinogenic drugs and haem.Biochem J. 1988 Oct 1;255(1):267-75. Biochem J. 1988. PMID: 3196319 Free PMC article.
-
Enzymatic defect in "X-linked" sideroblastic anemia: molecular evidence for erythroid delta-aminolevulinate synthase deficiency.Proc Natl Acad Sci U S A. 1992 May 1;89(9):4028-32. doi: 10.1073/pnas.89.9.4028. Proc Natl Acad Sci U S A. 1992. PMID: 1570328 Free PMC article.
-
On the mechanism of induction of chick embryo hepatic delta-aminolaevulinate synthase by translational blockers.Biochem J. 1991 Mar 1;274 ( Pt 2)(Pt 2):619-21. doi: 10.1042/bj2740619b. Biochem J. 1991. PMID: 2006926 Free PMC article. No abstract available.
-
Regulation of production of delta-aminolaevulinate synthase in tissues of chick embryos. Effects of porphyrogenic agents and of haem precursors.Biochem J. 1989 Sep 15;262(3):815-21. doi: 10.1042/bj2620815. Biochem J. 1989. PMID: 2590168 Free PMC article.
-
Cytochrome P450 regulation: the interplay between its heme and apoprotein moieties in synthesis, assembly, repair, and disposal.Drug Metab Rev. 2011 Feb;43(1):1-26. doi: 10.3109/03602532.2010.515222. Epub 2010 Sep 23. Drug Metab Rev. 2011. PMID: 20860521 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
