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. 2023 Dec;103(12):935-940.
doi: 10.1002/cyto.a.24797. Epub 2023 Oct 3.

OMIP-097: High-parameter phenotyping of human platelets by spectral flow cytometry

Benjamin E J Spurgeon  1 , Andrew L Frelinger 3rd  1
Affiliations

OMIP-097: High-parameter phenotyping of human platelets by spectral flow cytometry

Benjamin E J Spurgeon et al. Cytometry A. 2023 Dec.

Abstract

Using spectral flow cytometry, we developed a 16-color panel for analysis of platelet phenotype and function in human whole blood. The panel contains markers of clinical relevance and follows an optimized protocol for the high-parameter phenotyping of (phosphatidylserine positive) procoagulant platelets. Inclusion of established markers, such as CD62P and PAC-1, allows the subsetting of classic (proinflammatory and proaggregatory) phenotypes, while addition of novel markers, such as TLR9, allows the resolution of platelets with nonclassic functions. Multiple inducible (C3b, CD63, CD107a, CD154, and TLT-1) and constitutive (CD29, CD31, CD32, CD36, CD42a, CD61, and GPVI) markers are also measurable, and we demonstrate the use of automatic gating for platelet analysis. The panel is widely applicable to research and clinical settings and can be readily modified, should users wish to tailor the panel to more specific needs.

Keywords: biomarkers; blood platelets; flow cytometry; phenotype; platelet activation.

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References

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